catalog number :
MBS932564
products type :
ELISA Kit
products full name :
Bovine Retinol-binding protein 3, RBP3 ELISA Kit
products short name :
retinol binding protein 3, interstitial
products name syn :
Bovine Retinol-binding protein 3 (RBP3) ELISA kit; D10S64; D10S65; D10S66; IRBP; RBPI; interphotoreceptor retinoid-binding protein; retinol-binding protein 3; retinol-binding protein 3; interstitial; retinol binding protein 3; interstitial
other names :
retinol-binding protein 3; Retinol-binding protein 3; retinol-binding protein 3; IRBP; 7S protein; protein 7S; interstitial retinol-binding protein; interphotoreceptor retinoid-binding protein; Interphotoreceptor retinoid-binding protein; IRBP; Interstitial retinol-binding protein; Protein 7S
products gene name :
RBP3
other gene names :
RBP3; RBP3; IRBP
uniprot entry name :
RET3_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Bovine RBP3. No significant cross-reactivity or interference between Bovine RBP3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 3: Transport
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RBP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RBP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBP3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_776589.1
ncbi gb acc num :
NM_174164.1
ncbi mol weight :
139,698 Da
uniprot summary :
Function: IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina.
size4 :
10x96-Strip-Wells