Human Aldehyde dehydrogenase family 1 member A3, ALDH1A3 ELISA Kit | MyBioSource MBS932561 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Aldehyde dehydrogenase family 1 member A3, ALDH1A3 ELISA Kit

catalog :

MBS932561

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS932561

products type :

ELISA Kit

products full name :

Human Aldehyde dehydrogenase family 1 member A3, ALDH1A3 ELISA Kit

products short name :

aldehyde dehydrogenase 1 family, member A3

products name syn :

Human Aldehyde dehydrogenase family 1 member A3 (ALDH1A3) ELISA kit; ALDH1A6; ALDH6; RALDH3; acetaldehyde dehydrogenase 6; aldehyde dehydrogenase 1A3; aldehyde dehydrogenase 6; retinaldehyde dehydrogenase 3; aldehyde dehydrogenase 1 family; member A3

other names :

aldehyde dehydrogenase family 1 member A3 isoform 1; Aldehyde dehydrogenase family 1 member A3; aldehyde dehydrogenase family 1 member A3; aldehyde dehydrogenase 6; acetaldehyde dehydrogenase 6; retinaldehyde dehydrogenase 3; aldehyde dehydrogenase 1 family, member A3; Aldehyde dehydrogenase 6; Retinaldehyde dehydrogenase 3; RALDH-3; RalDH3

products gene name :

ALDH1A3

other gene names :

ALDH1A3; ALDH1A3; ALDH6; MCOP8; RALDH3; ALDH1A6; ALDH6; RALDH-3; RalDH3

uniprot entry name :

AL1A3_HUMAN

reactivity :

Human

sequence length :

512

specificity :

This assay has high sensitivity and excellent specificity for detection of human ALDH1A3. No significant cross-reactivity or interference between human ALDH1A3 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml -1500 pg/ml. Sensitivity: The minimum detectable dose of human ALDH1A3 is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ALDH1A3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ALDH1A3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALDH1A3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALDH1A3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

153266822

ncbi acc num :

NP_000684.2

ncbi gb acc num :

NM_000693.3

uniprot acc num :

P47895

ncbi mol weight :

56,108 Da

ncbi pathways :

Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427); Glycolysis / Gluconeogenesis Pathway (82926); Glycolysis / Gluconeogenesis Pathway (287); Histidine Metabolism Pathway (82958); Histidine Metabolism Pathway (324); Metabolic Pathways (132956); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (83031)

ncbi summary :

This gene encodes an aldehyde dehydrogenase enzyme that uses retinal as a substrate. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

uniprot summary :

ALDH1A3: Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Seems to be the key enzyme in the formation of an RA gradient along the dorso-ventral axis during the early eye development and also in the development of the olfactory system. Belongs to the aldehyde dehydrogenase family. Protein type: Amino Acid Metabolism - phenylalanine; EC 1.2.1.5; Xenobiotic Metabolism - metabolism by cytochrome P450; Amino Acid Metabolism - histidine; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Oxidoreductase; Amino Acid Metabolism - tyrosine. Chromosomal Location of Human Ortholog: 15q26.3. Cellular Component: cytoplasm; cytosol. Molecular Function: aldehyde dehydrogenase (NAD) activity; protein homodimerization activity; aldehyde dehydrogenase [NAD(P)+] activity; retinal dehydrogenase activity. Biological Process: inner ear morphogenesis; retinal metabolic process; positive regulation of apoptosis; embryonic eye morphogenesis; retinol metabolic process; righting reflex; olfactory pit development; retinoic acid metabolic process; optic cup morphogenesis involved in camera-type eye development; locomotory behavior; neuromuscular process controlling balance; nucleus accumbens development. Disease: Microphthalmia, Isolated 8

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage