Human Egl nine homolog 2, EGLN2 ELISA Kit | MyBioSource MBS9325014 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Egl nine homolog 2, EGLN2 ELISA Kit

catalog :

MBS9325014

quantity :

48-Strip-Wells

price :

470 USD

product information

catalog number :

MBS9325014

products type :

ELISA Kit

products full name :

Human Egl nine homolog 2, EGLN2 ELISA Kit

products short name :

[egl nine homolog 2 (C. elegans)]

products name syn :

[Human Egl nine homolog 2 (EGLN2) ELISA kit; DKFZp434E026; EIT6; HIFPH1; HPH-3; PHD1; EGL nine (C.elegans) homolog 2; HIF prolyl hydroxylase 1; HIF-prolyl hydroxylase 1; estrogen-induced tag 6; hypoxia-inducible factor prolyl hydroxylase 1; prolyl; egl nine homolog 2 (C. elegans)]

other names :

[egl nine homolog 2; Egl nine homolog 2; egl nine homolog 2; estrogen-induced tag 6; HIF-prolyl hydroxylase 1; hypoxia-inducible factor prolyl hydroxylase 1; prolyl hydroxylase domain-containing protein 1; egl-9 family hypoxia-inducible factor 2; Estrogen-induced tag 6; HPH-3; Hypoxia-inducible factor prolyl hydroxylase 1; HIF-PH1; HIF-prolyl hydroxylase 1; HPH-1; Prolyl hydroxylase domain-containing protein 1; PHD1]

products gene name :

[EGLN2]

other gene names :

[EGLN2; EGLN2; EIT6; PHD1; HPH-1; HPH-3; HIFPH1; HIF-PH1; EIT6; HIF-PH1; HIF-prolyl hydroxylase 1; HPH-1; PHD1]

uniprot entry name :

EGLN2_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between this analyte and analogues is observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Assay Type: Quantitative Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

products description :

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of EGLN2 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

ncbi gi num :

16604260

ncbi acc num :

NP_444274.1

ncbi gb acc num :

NM_053046.3

uniprot acc num :

Q96KS0

ncbi mol weight :

43,650 Da

ncbi pathways :

HIF-2-alpha Transcription Factor Network Pathway (137956); Pathways In Cancer (83105); Renal Cell Carcinoma Pathway (83107); Renal Cell Carcinoma Pathway (519)

ncbi summary :

The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. [provided by RefSeq, Feb 2011]

uniprot summary :

EGLN2: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. 2 isoforms of the human protein are produced by alternative initiation. Protein type: EC 1.14.11.29; Oxidoreductase. Chromosomal Location of Human Ortholog: 19q13.2. Cellular Component: nucleoplasm; cytosol; nucleus. Molecular Function: protein binding; oxygen sensor activity; L-ascorbic acid binding; ferrous iron binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; peptidyl-proline 4-dioxygenase activity. Biological Process: estrogen receptor signaling pathway; cell redox homeostasis; regulation of neuron apoptosis; positive regulation of protein catabolic process; response to hypoxia; regulation of cell growth; peptidyl-proline hydroxylation to 4-hydroxy-L-proline

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

680

size3 :

5x96-Strip-Wells

price3 :

3100

size4 :

10x96-Strip-Wells

price4 :

6095