Human Complement component C8 beta chain, C8B ELISA Kit | MyBioSource MBS932422 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Complement component C8 beta chain, C8B ELISA Kit

catalog :

MBS932422

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS932422

products type :

ELISA Kit

products full name :

Human Complement component C8 beta chain, C8B ELISA Kit

products short name :

complement component 8, beta polypeptide

products name syn :

Human Complement component C8 beta chain (C8B) ELISA kit; MGC163447; ; complement component 8; beta polypeptide

other names :

complement component C8 beta chain isoform 1 preproprotein; Complement component C8 beta chain; complement component C8 beta chain; complement component 8 subunit beta; complement component 8, beta polypeptide; Complement component 8 subunit beta

products gene name :

C8B

other gene names :

C8B; C8B; C82

uniprot entry name :

CO8B_HUMAN

reactivity :

Human

sequence length :

591

specificity :

This assay has high sensitivity and excellent specificity for detection of human C8B. No significant cross-reactivity or interference between human C8B and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 2.74 pg/ml-2000 pg/ml. Sensitivity: The minimum detectable dose of human C8B is typically less than 1.37 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human C8B concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C8B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C8B present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for C8B is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C8B bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

514239916

ncbi acc num :

NP_000057.2

ncbi gb acc num :

NM_000066.3

uniprot acc num :

P07358

ncbi mol weight :

67,047 Da

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Complement Activation, Classical Pathway (198823); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Immune System Pathway (106386); Innate Immune System Pathway (106387); Prion Diseases Pathway (101144); Prion Diseases Pathway (100065)

ncbi summary :

This gene encodes one of the three subunits of the complement component 8 (C8) protein. C8 is composed of equimolar amounts of alpha, beta and gamma subunits, which are encoded by three separate genes. C8 is one component of the membrane attack complex, which mediates cell lysis, and it initiates membrane penetration of the complex. This protein mediates the interaction of C8 with the C5b-7 membrane attack complex precursor. In humans deficiency of this protein is associated with increased risk of meningococcal infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

uniprot summary :

C8B: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Defects in C8B are a cause of complement component 8 deficiency type 2 (C8D2). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. Belongs to the complement C6/C7/C8/C9 family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p32.2. Cellular Component: membrane attack complex; extracellular space; membrane; extracellular region; vesicle. Molecular Function: protein complex binding. Biological Process: cytolysis; complement activation, alternative pathway; regulation of complement activation; innate immune response; immune response; complement activation, classical pathway; complement activation. Disease: Complement Component 8 Deficiency, Type Ii

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage