catalog number :
MBS932416
products type :
ELISA Kit
products full name :
Rat Voltage-dependent calcium channel subunit alpha-2/delta-1, CACNA2D1 ELISA Kit
products short name :
calcium channel, voltage-dependent, alpha 2/delta subunit 1
products name syn :
Rat Voltage-dependent calcium channel subunit alpha-2/delta-1 (CACNA2D1) ELISA kit; H_DJ0560O14.1; CACNA2; CACNL2A; CCHL2A; calcium channel; L type; alpha 2 polypeptide; dihydropyridine-sensitive L-type; calcium channel alpha-2/delta subunit; calcium channel; voltage-dependent; alpha 2/delta subunit 1
other names :
voltage-dependent calcium channel subunit alpha-2/delta-1 preproprotein; Voltage-dependent calcium channel subunit alpha-2/delta-1; voltage-dependent calcium channel subunit alpha-2/delta-1; voltage-gated calcium channel subunit alpha-2/delta-1; dihydropryridine-sensitive calcium channel alpha-2 subunit; Voltage-gated calcium channel subunit alpha-2/delta-1Cleaved into the following 2 chains:Voltage-dependent calcium channel subunit alpha-2-1; Voltage-dependent calcium channel subunit delta-1
products gene name :
CACNA2D1
other gene names :
CACNA2D1; CACNA2D1; CACNL2A; CCHL2A
uniprot entry name :
CA2D1_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat CACNA2D1. No significant cross-reactivity or interference between rat CACNA2D1and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of rat CACNA2D1 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CACNA2D1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CACNA2D1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CACNA2D1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CACNA2D1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075745.1
ncbi gb acc num :
NM_001082276.1
ncbi mol weight :
125,043 Da
uniprot summary :
Function: The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Plays an important role in excitation-contraction coupling.
size4 :
10x96-Strip-Wells