ELISA/assay

Mouse 80 kDa MCM3-associated protein, MCM3AP ELISA Kit

MBS9323977

48-Strip-Wells

470 USD

ELISA Kit

Mouse 80 kDa MCM3-associated protein, MCM3AP ELISA Kit

[minichromosome maintenance complex component 3 associated protein]

[Mouse 80 kDa MCM3-associated protein (MCM3AP) ELISA kit; FLJ44336; FLJ45306; GANP; KIAA0572; MAP80; MCM3 import protein; MCM3 minichromosome maintenance deficient 3 associated protein; OTTHUMP00000115776; germinal center-associated nuclear protein; minichromo; minichromosome maintenance complex component 3 associated protein]

[germinal-center associated nuclear protein; Germinal-center associated nuclear protein; germinal-center associated nuclear protein; nuclear protein GANP; GC-associated DNA primase; 80 kDa MCM3-associated protein; minichromosome maintenance deficient 3-associated protein; minichromosome maintenance deficient 3 (S. cerevisiae) associated protein; GC-associated DNA primase]

[MCM3AP]

[Mcm3ap; Mcm3ap; GANP; mKIAA0572; Ganp; Map80; GANP]

GANP_MOUSE

Mouse

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. Assay Type: Sandwich (Quantitative). Detection Range: 3.12ng/ml-100ng/ml. Sensitivity: 1.0ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of MCM3AP (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

109627648

NP_062307.2

NM_019434.2

Q9WUU9

217,363 Da

GANP: is a member of the SAC3 family of nuclear export factors. In combination with ENY2, PCID2 and centrins, forms the TREX-2 complex that links transcription/ processing with nuclear mRNA export. GANP, required to recruit ENY2 to nuclear pore complexes (NPCs), requires both its CID and MCM3AP domains, together with nucleoporin Nup153, for recruitment to NPCs. The MCM3AP domain, corresponding to the carboxy-terminal 721 amino acids of GANP, shows homology with the Gcn5-related N acetyltransferase superfamily. The protein MCM3AP is encoded entirely within the 3¿ region of the GANP gene. MCM3AP transcription begins within intron 16 of GANP immediately upstream of the first MCM3AP exon, where NF-IL6, AP-1, NF-¿B, and p53 consensus transcription factor binding sites are located. In contrast, the GANP promoter region contains consensus transcription factor binding sites for E2F, E-box, BSAP, and PU.1. MCM3AP interacts with MCM3, which it can acetylate weakly in vivo. GANP is required for efficient mRNA nuclear export: GANP depletion results in the nuclear accumulation of poly(A)+RNA. It interacts via its N-terminal FG repeat domain with the mRNA export factor NXF1. GANP plays a role in the immune response: antibodies from mice deficient for GANP have reduced affinities against T-cell dependent antigens and a lower frequency of variable region somatic mutation. GANP expression is markedly decreased in malignant glioma (MG) with a poor prognosis, consistently lower than that observed in anaplastic astrocytomas. Significant decreases in GANP expression are associated with hyperploidy and chromosomal instability. Protein type: Karyopherin; Acetyltransferase. Cellular Component: nuclear membrane; cytoplasm; nuclear pore; nucleus; cytosol. Biological Process: protein transport; mRNA transport; immune system process; transport

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095