catalog number :
MBS9323768
products type :
ELISA Kit
products full name :
Mouse MAP kinase-activating death domain protein, MADD ELISA Kit
products short name :
[MAP-kinase activating death domain]
products name syn :
[Mouse MAP kinase-activating death domain protein (MADD) ELISA kit; DENN; FLJ35600; FLJ36300; IG20; KIAA0358; RAB3GEP; MAP-kinase activating death domain-containing protein; OTTHUMP00000197132; OTTHUMP00000197133; OTTHUMP00000197138; OTTHUMP00000197139; Rab3 GDP/GTP exch; MAP-kinase activating death domain]
other names :
[MAP kinase-activating death domain protein isoform 10; MAP kinase-activating death domain protein; MAP kinase-activating death domain protein; Rab3 GEP; rab3 GDP/GTP exchange factor; Rab3 GDP/GTP exchange protein; insulinoma-glucagonoma protein 20; MAP-kinase activating death domain; Rab3 GDP/GTP exchange factor]
products gene name :
[MADD]
other gene names :
[Madd; Madd; IG20; 9630059K23Rik; Kiaa0358]
uniprot entry name :
MADD_MOUSE
specificity :
No significant cross-reactivity or interference between this analyte and analogues is observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. Assay Type: Sandwich (Quantitative). Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of MADD (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
ncbi acc num :
NP_001171190.1
ncbi gb acc num :
NM_001177719.1
ncbi mol weight :
175,180 Da
uniprot summary :
MADD: a guanine nucleotide exchange factor (GEF) that activates RAB3A/C/D. A GEF for Rab27A/B in melanocytes and during isoproterenol (IPR)-stimulated amylase release from rat parotid acinar cells. Plays a significant role in regulating cell proliferation, survival and death through alternatively spliced isoforms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis). Interacts with the death domain of TNFRSF1A through its own death domain. Highly expressed in fetal brain and kidney, adult testis, ovary, brain and heart. Overexpression of MADD activates the mitogen-activated protein (MAP) kinase extracellular signal-regulated kinase (ERK). Expression of the MADD death domain stimulates both the ERK and c-JUN N-terminal kinase MAP kinases and induces the phosphorylation of cytosolic phospholipase A2. Isoform 1 is pro-apoptotic, isoform 5 is anti-apoptotic and isoform 3 and isoform 4 have no effect. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines. Isoform 5 is associated with increased cell proliferation and isoform 2 with decreased proliferation. Belongs to the MADD family. Seven isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; GEFs, Rab; Adaptor/scaffold; GEFs. Cellular Component: synaptic vesicle; membrane; cytoplasm; integral to membrane; plasma membrane; synapse; nucleus; cytosol. Molecular Function: guanyl-nucleotide exchange factor activity; Rab guanyl-nucleotide exchange factor activity. Biological Process: regulation of Rab protein signal transduction; negative regulation of growth hormone secretion; regulation of apoptosis; apoptosis; regulation of cell cycle; activation of MAPK activity; positive regulation of GTPase activity
size4 :
10x96-Strip-Wells