ELISA/assay

Human Cold-inducible RNA-binding protein, CIRBP ELISA Kit

MBS932362

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human Cold-inducible RNA-binding protein, CIRBP ELISA Kit

[cold inducible RNA binding protein]

[Human Cold-inducible RNA-binding protein (CIRBP) ELISA kit; CIRP; Cold-inducible RNA-binding protein; cold inducible RNA-binding protein; glycine-rich RNA binding protein; cold inducible RNA binding protein]

[cold-inducible RNA-binding protein isoform 1; Cold-inducible RNA-binding protein; cold-inducible RNA-binding protein; A18 hnRNP; glycine-rich RNA binding protein; cold inducible RNA-binding protein; cold inducible RNA binding protein; A18 hnRNP; Glycine-rich RNA-binding protein CIRP]

[CIRBP]

[CIRBP; CIRBP; CIRP; A18HNRNP; CIRP]

CIRBP_HUMAN

Human

172

This assay has high sensitivity and excellent specificity for detection of mouse CIC. No significant cross-reactivity or interference between mouse CIC and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma. Assay Type: Quantitative Sandwich. Detection Range: 15.6 ng/ml-1000 ng/ml. Sensitivity: 3.9 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CIC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CIC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CIC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CIC bound in the initial step. The color development is stopped and the intensity of the color is measured.

4502847

NP_001271.1

NM_001280.2

Q14011

18,648 Da

Function: Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor Promotes assembly of stress granules (SGs), when overexpressed.2 PublicationsManual assertion based on experiment in:Ref.5

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145