catalog number :
MBS932294
products type :
ELISA Kit
products full name :
Mouse Growth/differentiation factor 8, MSTN ELISA Kit
products short name :
[myostatin]
products name syn :
[Mouse Growth/differentiation factor 8 (MSTN) ELISA kit; GDF8; growth differentiation factor 8; myostatin]
other names :
[growth/differentiation factor 8; Growth/differentiation factor 8; growth/differentiation factor 8; GDF-8; compact; growth differentiation factor 8; myostatin; Myostatin]
products gene name :
[MSTN]
other gene names :
[Mstn; Mstn; Cmpt; Gdf8; Gdf8; GDF-8]
uniprot entry name :
GDF8_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse MSTN. No significant cross-reactivity or interference between mouse MSTN and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of mouse MSTN is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MSTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MSTN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MSTN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MSTN bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_034964.1
ncbi gb acc num :
NM_010834.2
ncbi mol weight :
42,921 Da
ncbi pathways :
Cardiac Hypertrophy: MiR-208 Pathway (198343); Hypertrophy Model Pathway (198326)
uniprot summary :
MSTN: Acts specifically as a negative regulator of skeletal muscle growth. Defects in MSTN are the cause of muscle hypertrophy (MSLHP). MSLHP is a condition characterized by increased muscle bulk and strength. Affected individuals are exceptionally strong. Belongs to the TGF-beta family. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; cytoplasm; extracellular region. Molecular Function: heparin binding; identical protein binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding; receptor binding. Biological Process: regulation of apoptosis; negative regulation of muscle hypertrophy; transforming growth factor beta receptor signaling pathway; positive regulation of transcription, DNA-dependent; regulation of MAPKKK cascade; negative regulation of skeletal muscle growth; cell development; growth
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells