Human Nesprin-2, SYNE2 ELISA Kit | MyBioSource MBS932261 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Nesprin-2, SYNE2 ELISA Kit

catalog :

MBS932261

quantity :

48-Strip-Wells

price :

510 USD

product information

catalog number :

MBS932261

products type :

ELISA Kit

products full name :

Human Nesprin-2, SYNE2 ELISA Kit

products short name :

spectrin repeat containing, nuclear envelope 2

products name syn :

Human Nesprin-2 (SYNE2) ELISA kit; DKFZp434H2235; DKFZp686E01115; DKFZp686H1931; EDMD5; FLJ11014; FLJ43727; FLJ45710; FLJ46790; KIAA1011; NUA; NUANCE; Nesprin-2; SYNE-2; nesprin 2; nucleus and actin connecting element; synaptic nuclei; spectrin repeat containing; nuclear envelope 2

other names :

nesprin-2 isoform 1; Nesprin-2; nesprin-2; nesprin 2; polytrophin; synaptic nuclei expressed gene 2; synaptic nuclear envelope protein 2; nuclear envelope spectrin repeat protein 2; nucleus and actin connecting element protein; spectrin repeat containing, nuclear envelope 2; Nuclear envelope spectrin repeat protein 2; Nucleus and actin connecting element protein; Protein NUANCE; Synaptic nuclear envelope protein 2; Syne-2

products gene name :

SYNE2

other gene names :

SYNE2; SYNE2; NUA; EDMD5; Nesp2; TROPH; NUANCE; SYNE-2; Nesprin-2; KIAA1011; NUA; Protein NUANCE; Syne-2

uniprot entry name :

SYNE2_HUMAN

reactivity :

Human

sequence length :

6885

specificity :

This assay has high sensitivity and excellent specificity for detection of Human SYNE2. No significant cross-reactivity or interference between Human SYNE2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.5 pg/ml-1500 pg/ml. Sensitivity: 5.8 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SYNE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SYNE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNE2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

118918403

ncbi acc num :

NP_055995.4

ncbi gb acc num :

NM_015180.4

uniprot acc num :

Q8WXH0

ncbi mol weight :

796,442 Da

ncbi pathways :

Cell Cycle Pathway (530733); Meiosis Pathway (477133); Meiotic Synapsis Pathway (477134); Ovarian Infertility Genes Pathway (198801)

ncbi summary :

The protein encoded by this gene is a nuclear outer membrane protein that binds cytoplasmic F-actin. This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

uniprot summary :

SYNE2: a nuclear transmembrane protein involved in the maintenance of nuclear organization and structural integrity. The largest part of the protein is cytoplasmic, while its C-terminal part is associated with the nuclear envelope, most probably the outer nuclear membrane. Remains associated with the nuclear envelope during its breakdown in mitotic cells. Probable anchoring protein which theters the nucleus to the cytoskeleton. Connects nuclei to the cytoskeleton by interacting with the nuclear envelope and with F-actin in the cytoplasm. Nine alternatively spliced isoforms have been described. Protein type: Adaptor/scaffold; Membrane protein, integral. Chromosomal Location of Human Ortholog: 14q23.2. Cellular Component: nuclear outer membrane; filopodium membrane; intermediate filament cytoskeleton; sarcoplasmic reticulum membrane; nuclear membrane; focal adhesion; nuclear lumen; sarcoplasmic reticulum; mitochondrion; integral to membrane; nuclear envelope; Z disc; nucleoplasm; cytoplasm; nucleus. Molecular Function: actin filament binding; protein binding; actin binding. Biological Process: centrosome localization; establishment and/or maintenance of cell polarity; nuclear migration along microfilament; nuclear migration; nuclear membrane organization and biogenesis; positive regulation of cell migration. Disease: Emery-dreifuss Muscular Dystrophy 5, Autosomal Dominant

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800