catalog number :
MBS932256
products type :
ELISA Kit
products full name :
Human Spexin, C12orf39 ELISA Kit
products short name :
chromosome 12 open reading frame 39
products name syn :
Human Spexin (C12orf39) ELISA kit; MGC10946; spexin; chromosome 12 open reading frame 39
other names :
spexin; Spexin; spexin; NPQ; neuropeptide Q; spexin hormone; NPQ; Neuropeptide Q; Spexin hormoneCleaved into the following 2 chains:Spexin-1; Spexin-2Alternative name(s):NPQ 53-70
products gene name :
C12orf39
other gene names :
SPX; SPX; C12orf39; C12orf39
uniprot entry name :
SPXN_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human C12orf39. No significant cross-reactivity or interference between human C12orf39 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 4000 pg/ml. Sensitivity: The minimum detectable dose of human C12orf39 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C12orf39 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C12orf39 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C12orf39 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C12orf39 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_085049.1
ncbi gb acc num :
NM_030572.2
ncbi mol weight :
13,302 Da
uniprot summary :
SPX: Induces contraction of stomach muscle. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 12p12.1. Cellular Component: extracellular space; transport vesicle. Molecular Function: neuropeptide hormone activity; type 3 galanin receptor binding; type 2 galanin receptor binding. Biological Process: negative regulation of heart rate; positive regulation of systemic arterial blood pressure; positive regulation of transcription from RNA polymerase II promoter; regulation of sensory perception of pain; negative regulation of appetite
size4 :
10x96-Strip-Wells