Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit | MyBioSource MBS9321752 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit

catalog :

MBS9321752

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS9321752

products type :

ELISA Kit

products full name :

Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit

products short name :

Tryptase alpha/beta-1 (TPSAB1)

products name syn :

tryptase alpha/beta 1; TPS1; TPS2; TPSB1; mast cell alpha II tryptase mast cell beta I tryptase tryptase alpha II tryptase beta 1 tryptase beta I tryptase-I tryptase-III

other names :

tryptase alpha/beta-1; Tryptase alpha/beta-1; tryptase alpha/beta-1; tryptase I; tryptase-1; tryptase-I; tryptase-III; tryptase beta 1; tryptase beta I; tryptase beta-1; tryptase alpha-1; tryptase alpha II; mast cell beta I tryptase; mast cell alpha II tryptase; tryptase alpha/beta 1; Tryptase I; Tryptase alpha-1

products gene name :

TPSAB1

other gene names :

TPSAB1; TPSAB1; TPS1; TPS2; TPSB1; TPS1; TPS2; TPSB1; Tryptase-1

uniprot entry name :

TRYB1_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human TPSAB1 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TPSAB1 concentrations in Human serum, plasma and other body fluids. Using Purified Human TPSAB1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TPSAB1 and TPSAB1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TPSAB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TPSAB1 concentrations in Human serum, plasma and other body fluids. Using Purified Human TPSAB1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TPSAB1 and TPSAB1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TPSAB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

13775595

ncbi acc num :

NP_003285.2

ncbi gb acc num :

NM_003294.3

uniprot acc num :

Q15661

ncbi mol weight :

30,515 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

uniprot summary :

TPSAB1: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Belongs to the peptidase S1 family. Tryptase subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.21.59; Secreted, signal peptide; Protease; Secreted. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: extracellular matrix; extracellular space; extracellular region. Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; defense response; proteolysis

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage