product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Spondin-1, SPON1 ELISA Kit
catalog :
MBS932153
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS932153
products type :
ELISA Kit
products full name :
Human Spondin-1, SPON1 ELISA Kit
products short name :
spondin 1, extracellular matrix protein
products name syn :
Human Spondin-1 (SPON1) ELISA kit; KIAA0762; MGC10724; f-spondin; VSGP/F-spondin; spondin 1; (f-spondin) extracellular matrix protein; spondin 1; extracellular matrix protein
other names :
spondin-1; Spondin-1; spondin-1; spondin 1, (f-spondin) extracellular matrix protein; vascular smooth muscle cell growth-promoting factor; spondin 1, extracellular matrix protein; F-spondin; Vascular smooth muscle cell growth-promoting factor
products gene name :
SPON1
other gene names :
SPON1; SPON1; f-spondin; VSGP/F-spondin; KIAA0762; VSGP
uniprot entry name :
SPON1_HUMAN
reactivity :
Human
sequence length :
807
specificity :
This assay has high sensitivity and excellent specificity for detection of Human SPON1. No significant cross-reactivity or interference between Human SPON1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for spondin-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any spondin-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for spondin-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of spondin-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
110347423
ncbi acc num :
NP_006099.2
ncbi gb acc num :
NM_006108.3
uniprot acc num :
Q9HCB6
ncbi mol weight :
90,973 Da
ncbi pathways :
Metabolism Of Proteins Pathway (106230); O-glycosylation Of TSR Domain-containing Proteins Pathway (980460); O-linked Glycosylation Pathway (980459); Post-translational Protein Modification Pathway (161012)
uniprot summary :
SPON1: Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11p15.2. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; endoplasmic reticulum lumen. Molecular Function: protein binding. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; post-translational protein modification; cell adhesion
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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