ELISA/assay

Human Immunity-related GTPase family M protein, IRGM ELISA Kit

MBS9320915

48-Strip-Wells

435 USD

ELISA Kit

Human Immunity-related GTPase family M protein, IRGM ELISA Kit

immunity-related GTPase family, M

Human Immunity-related GTPase family M protein (IRGM) ELISA kit; IFI1; IRGM1; LRG-47; LRG47; MGC149263; MGC149264; LRG-47-like protein; immunity-related GTPase M; immunity-related GTPase family; M1; immunity-related GTPase family; M

immunity-related GTPase family M protein; Immunity-related GTPase family M protein; immunity-related GTPase family M protein; LRG-47-like protein; interferon-inducible protein 1; immunity-related GTPase family, M1; LPS-stimulated RAW 264.7 macrophage protein 47 homolog; immunity-related GTPase family, M; Immunity-related GTPase family M protein 1; Interferon-inducible protein 1; LPS-stimulated RAW 264.7 macrophage protein 47 homolog; LRG-47

IRGM

IRGM; IRGM; IFI1; IRGM1; LRG47; LRG-47; IFI1; IRGM1; LRG47; LRG-47

IRGM_HUMAN

Human

No significant cross-reactivity or interference between Human IRGM and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IRGM concentrations in Human serum, plasma and other body fluids. Using Purified Human IRGM antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IRGM and IRGM antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IRGM in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IRGM concentrations in Human serum, plasma and other body fluids. Using Purified Human IRGM antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IRGM and IRGM antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IRGM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

224809576

NP_001139277.1

NM_001145805.1

A1A4Y4

20,142 Da

Toxoplasmosis Pathway (169642); Toxoplasmosis Pathway (169493)

This gene encodes a member of the p47 immunity-related GTPase family. The encoded protein may play a role in the innate immune response by regulating autophagy formation in response to intracellular pathogens. Polymorphisms that affect the normal expression of this gene are associated with a susceptibility to Crohn's disease and tuberculosis.[provided by RefSeq, Oct 2010]

IRGM: Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility. Defects in IRGM are the cause of susceptibility to inflammatory bowel disease type 19 (IBD19). A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Belongs to the interferon-inducible GTPase family. Protein type: Hydrolase; Endoplasmic reticulum; EC 3.6.5.-. Chromosomal Location of Human Ortholog: 5q33.1. Cellular Component: Golgi membrane; phagocytic vesicle membrane; cell projection; phagocytic cup. Molecular Function: GTP binding; hydrolase activity. Biological Process: innate immune response; autophagy; inflammatory response. Disease: Mycobacterium Tuberculosis, Susceptibility To; Inflammatory Bowel Disease 19

48-Strip-Wells

435 USD

96-Strip-Wells

600