catalog number :
MBS932065
products type :
ELISA Kit
products full name :
Human Protein FAM13A, FAM13A ELISA Kit
products short name :
family with sequence similarity 13, member A
products name syn :
Human Protein FAM13A (FAM13A) ELISA kit; FAM13A1; FLJ34562; MGC105131; FAM13A1_v2 protein; family with sequence similarity 13; member A1; family with sequence similarity 13; member A
other names :
protein FAM13A; Protein FAM13A; protein FAM13A; protein Precm1; family with sequence similarity 13, member A1; family with sequence similarity 13, member A; Protein Precm1
products gene name :
FAM13A
other gene names :
FAM13A; FAM13A; PRECM1; FAM13A1; FAM13A1; PRECM1
uniprot entry name :
FA13A_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of human FAM13A. No significant cross-reactivity or interference between human FAM13A and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml -1500 pg/ml. Sensitivity: The minimum detectable dose of human FAM13A is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FAM13A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FAM13A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAM13A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAM13A bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_777117.1
ncbi gb acc num :
NM_174692.2
ncbi mol weight :
80,099 Da
ncbi pathways :
Rho GTPase Cycle Pathway (1008531); Signal Transduction Pathway (1008398); Signaling By Rho GTPases Pathway (1008530)
size4 :
10x96-Strip-Wells