ELISA/assay

Rat Securin, PTTG1 ELISA Kit

MBS9319751

48-Strip-Wells

470 USD

ELISA Kit

Rat Securin, PTTG1 ELISA Kit

[pituitary tumor-transforming 1]

[Rat Securin (PTTG1) ELISA kit; EAP1; HPTTG; MGC126883; MGC138276; PTTG; TUTR1; ESP1-associated protein 1; OTTHUMP00000160845; pituitary tumor-transforming protein 1; securin; tumor-transforming protein 1; pituitary tumor-transforming 1]

[securin; Securin; securin; Pituitary tumor transforming; pituitary tumor-transforming gene 1 protein; pituitary tumor-transforming 1; Pituitary tumor-transforming gene 1 protein]

[PTTG1]

[Pttg1; Pttg1; Pttg; Pttg]

PTTG1_RAT

Rat

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Undiluted original Rat body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances. Assay Type: Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of PTTG1 (hereafter termed "analyte") in undiluted original Rat body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

11761541

NP_071786.1

NM_022391.2

P97613

21,571 Da

APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (573742); APC/C:Cdc20 Mediated Degradation Of Securin Pathway (573750); APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (573748); APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway (573752); Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (573746); Cell Cycle Pathway (573693); Cell Cycle, Mitotic Pathway (573715); Cell Cycle Pathway (198428); Cell Cycle Pathway (83446); Cell Cycle Pathway (463)

Securin: Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation. Belongs to the securin family. Protein type: Cell cycle regulation; Oncoprotein. Cellular Component: cytoplasm; nucleus. Molecular Function: heat shock protein binding; ribosome binding; cysteine protease inhibitor activity; SH3 domain binding. Biological Process: negative regulation of cell proliferation; mitosis; homologous chromosome segregation; cell division; regulation of cell growth; DNA repair; chromosome organization and biogenesis; chromosome segregation

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095