catalog number :
MBS9319185
products type :
ELISA Kit
products full name :
Mouse Hypoxia-inducible factor 3-alpha, HIF3A ELISA Kit
products short name :
[hypoxia inducible factor 3, alpha subunit]
products name syn :
[Mouse Hypoxia-inducible factor 3-alpha (HIF3A) ELISA kit; HIF-3A; HIF-3A2; HIF-3A4; IPAS; MOP7; PASD7; bHLHe17; hypoxia-inducible factor-3 alpha 4; inhibitory PAS domain protein; hypoxia inducible factor 3; alpha subunit]
other names :
[hypoxia-inducible factor 3-alpha isoform 2; Hypoxia-inducible factor 3-alpha; hypoxia-inducible factor 3-alpha; HIF3-alpha; HIF-3-alpha; HIF3-alpha-1; member of PAS protein 7; neonatal and embryonic PAS; inhibitory PAS domain protein; hypoxia inducible factor three alpha; basic-helix-loop-helix-PAS protein MOP7; hypoxia inducible factor 3, alpha subunit; Basic-helix-loop-helix-PAS protein MOP7; HIF3-alpha-1; Inhibitory PAS domain protein; IPAS; Member of PAS protein 7]
products gene name :
[HIF3A]
other gene names :
[Hif3a; Hif3a; Ipas; MOP7; NEPAS; bHLHe17; Mop7; HIF-3-alpha; HIF3-alpha; IPAS]
uniprot entry name :
HIF3A_MOUSE
specificity :
No significant cross-reactivity or interference between this analyte and analogues is observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Assay Type: Quantitative Sandwich. Detection Range: 6.25 pg/ml - 200 pg/ml. Sensitivity: 1.0 pg/ml
other info2 :
Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of HIF3A (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
ncbi acc num :
NP_058564.2
ncbi gb acc num :
NM_016868.3
ncbi mol weight :
73,044 Da
uniprot summary :
HIF3A: Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5 -TACGTG-3 within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Tumor suppressor; Transcription factor. Cellular Component: mitochondrion; cytoplasm; nuclear speck; nucleus. Molecular Function: protein dimerization activity; protein binding; signal transducer activity; DNA binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; signal transduction
size4 :
10x96-Strip-Wells