ELISA/assay

Rat Prestin, SLC26A5 ELISA Kit

MBS9319018

48-Strip-Wells

435 USD

ELISA Kit

Rat Prestin, SLC26A5 ELISA Kit

solute carrier family 26, member 5 (prestin)

Rat Prestin (SLC26A5) ELISA kit; DFNB61; MGC118886; MGC118887; MGC118888; MGC118889; PRES; deafness; neurosensory; autosomal recessive; 61; prestin; prestin (motor protein) ; solute carrier family 26; member 5 (prestin)

prestin; Prestin; prestin; prestin (motor protein); solute carrier family 26 member 5; solute carrier family 26, member 5 (prestin); solute carrier family 26 (anion exchanger), member 5; Solute carrier family 26 member 5

SLC26A5

Slc26a5; Slc26a5; Pres; prestin; Pres

S26A5_RAT

Rat

No significant cross-reactivity or interference between Rat SLC26A5 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 31.2pg/ml-1000pg/ml. Sensitivity: 5.0pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SLC26A5 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat SLC26A5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SLC26A5 and SLC26A5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SLC26A5 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SLC26A5 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat SLC26A5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SLC26A5 and SLC26A5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SLC26A5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

13540646

NP_110467.1

NM_030840.1

Q9EPH0

81,279 Da

molecular motor protein; involved in the electromechanical properties of hearing [RGD, Feb 2006]

SLC26A5: Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage- to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site. Defects in SLC26A5 are the cause of deafness autosomal recessive type 61 (DFNB61). A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the SLC26A/SulP transporter (TC 2.A.53) family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Motor; Transporter; Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family. Cellular Component: basolateral plasma membrane; cytoplasm; integral to membrane; plasma membrane; lateral plasma membrane. Molecular Function: protein homodimerization activity; spectrin binding; secondary active sulfate transmembrane transporter activity; motor activity; transcription factor binding. Biological Process: response to drug; regulation of cell shape; regulation of membrane potential; sensory perception of sound; positive regulation of cell size; response to salicylic acid stimulus; metabolic process; fructose transport; protein tetramerization

48-Strip-Wells

435 USD

96-Strip-Wells

600