ELISA/assay

Human Ubiquitin-conjugating enzyme E2 N, UBE2N ELISA Kit

MBS9318911

48-Strip-Wells

470 USD

ELISA Kit

Human Ubiquitin-conjugating enzyme E2 N, UBE2N ELISA Kit

[ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)]

[Human Ubiquitin-conjugating enzyme E2 N (UBE2N) ELISA kit; MGC131857; MGC8489; UBC13; UbcH-ben; bendless-like ubiquitin conjugating enzyme; ubiquitin carrier protein N; ubiquitin-conjugating enzyme E2N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC; ubiquitin-conjugating enzyme E2N (UBC13 homolog; yeast)]

[ubiquitin-conjugating enzyme E2 N; Ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2 N; yeast UBC13 homolog; ubiquitin-protein ligase N; ubiquitin carrier protein N; epididymis secretory protein Li 71; bendless-like ubiquitin conjugating enzyme; bendless-like ubiquitin-conjugating enzyme; ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N; Bendless-like ubiquitin-conjugating enzyme; Ubc13; UbcH13; Ubiquitin carrier protein N; Ubiquitin-protein ligase N]

[UBE2N]

[UBE2N; UBE2N; UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13; BLU]

UBE2N_HUMAN

Human

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances. Assay Type: Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of UBE2N (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

4507793

NP_003339.1

NM_003348.3

P61088

17,138 Da

Activated TLR4 Signalling Pathway (106400); Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Cytokine Signaling In Immune System Pathway (366171); Downstream TCR Signaling Pathway (106418); IL1-mediated Signaling Events Pathway (137944); IRAK1 Recruits IKK Complex Pathway (187087); IRAK1 Recruits IKK Complex Upon TLR7/8 Or 9 Stimulation Pathway (187083)

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq, Jul 2008]

UBE2N: The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical Lys-63 -linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the Lys-63 -linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the Lys-63 -linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Heterodimer with UBE2V2. Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific Lys-63 -linked polyubiquitination activity. Interacts with RNF8 and RNF168. Interacts with RNF11. Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the Lys-63 -linked polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair. Interacts with ARIH2 (via RING-type 2). Belongs to the ubiquitin-conjugating enzyme family. Protein type: Ubiquitin conjugating system; Ubiquitin ligase; DNA repair, damage; EC 6.3.2.19; Ligase. Chromosomal Location of Human Ortholog: 12q22. Cellular Component: protein complex; cytoplasm; nucleus; cytosol; UBC13-MMS2 complex; ubiquitin ligase complex. Molecular Function: protein binding; ubiquitin binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity. Biological Process: ubiquitin-dependent protein catabolic process; regulation of histone ubiquitination; positive regulation of DNA repair; positive regulation of I-kappaB kinase/NF-kappaB cascade; postreplication repair; positive regulation of histone modification; cytokine and chemokine mediated signaling pathway; DNA double-strand break processing; protein ubiquitination; protein modification process; proteolysis; T cell receptor signaling pathway; double-strand break repair via homologous recombination; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; regulation of DNA repair; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of ubiquitin-protein ligase activity; histone ubiquitination; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095