Human Lysosomal acid lipase/cholesteryl ester hydrolase, LIPA ELISA Kit | MyBioSource MBS931887 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Lysosomal acid lipase/cholesteryl ester hydrolase, LIPA ELISA Kit

catalog :

MBS931887

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS931887

products type :

ELISA Kit

products full name :

Human Lysosomal acid lipase/cholesteryl ester hydrolase, LIPA ELISA Kit

products short name :

[lipase A, lysosomal acid, cholesterol esterase]

products name syn :

[Human Lysosomal acid lipase/cholesteryl ester hydrolase (LIPA) ELISA kit; CESD; LAL; OTTHUMP00000020068; cholesterol ester hydrolase; lipase A; lysosomal acid lipase; sterol esterase; lipase A; lysosomal acid; cholesterol esterase]

other names :

[lysosomal acid lipase/cholesteryl ester hydrolase isoform 1; Lysosomal acid lipase/cholesteryl ester hydrolase; lysosomal acid lipase/cholesteryl ester hydrolase; sterol esterase; cholesteryl esterase; cholesterol ester hydrolase; acid cholesteryl ester hydrolase; lipase A, lysosomal acid, cholesterol esterase; Cholesteryl esterase; Lipase A; Sterol esterase]

products gene name :

[LIPA]

other gene names :

[LIPA; LIPA; LAL; CESD; Acid cholesteryl ester hydrolase; LAL]

uniprot entry name :

LICH_HUMAN

reactivity :

Human

sequence length :

399

specificity :

This assay has high sensitivity and excellent specificity for detection of sheep LH. No significant cross-reactivity or interference between sheep LH and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 2 mIU/ml-75 mIU/ml. Sensitivity: 0.5 mIU/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for LH. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LH bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

51317399

ncbi acc num :

NP_000226.2

ncbi gb acc num :

NM_000235.3

uniprot acc num :

P38571

ncbi mol weight :

45,419 Da

ncbi pathways :

Lysosome Pathway (99052); Lysosome Pathway (96865); Phase I, Non P450 Pathway (198854); Steroid Biosynthesis Pathway (82937); Steroid Biosynthesis Pathway (298)

ncbi summary :

This gene encodes lipase A, the lysosomal acid lipase (also known as cholesterol ester hydrolase). This enzyme functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides. Mutations in this gene can result in Wolman disease and cholesteryl ester storage disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014]

uniprot summary :

LIPA: Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor- mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation. Defects in LIPA are the cause of Wolman disease (WOD). WOD is a severe manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. WOD occurs in infancy and is nearly always fatal before the age of 1 year. Defects in LIPA are the cause of cholesteryl ester storage disease (CESD). CESD is a mild manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. It is characterized by late-onset. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.1.13; Lipid Metabolism - steroid biosynthesis; Hydrolase. Chromosomal Location of Human Ortholog: 10q23.2-q23.3. Cellular Component: lysosome. Molecular Function: sterol esterase activity; lipase activity. Biological Process: cell proliferation; homeostasis of number of cells within a tissue; tissue remodeling; cell morphogenesis; cytokine production; inflammatory response; lipid catabolic process; lung development. Disease: Lysosomal Acid Lipase Deficiency

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145

more info or order :

MyBioSource product webpage