Rat Tyrosine-protein phosphatase non-receptor type 6, PTPN6 ELISA Kit | MyBioSource MBS931885 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Tyrosine-protein phosphatase non-receptor type 6, PTPN6 ELISA Kit

catalog :

MBS931885

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS931885

products type :

ELISA Kit

products full name :

Rat Tyrosine-protein phosphatase non-receptor type 6, PTPN6 ELISA Kit

products short name :

protein tyrosine phosphatase, non-receptor type 6

products name syn :

Rat Tyrosine-protein phosphatase non-receptor type 6 (PTPN6) ELISA kit; HCP; HCPH; HPTP1C; PTP-1C; SH-PTP1; SHP-1; SHP-1L; SHP1; hematopoietic cell phosphatase; hematopoietic cell protein-tyrosine phosphatase; protein-tyrosine phosphatase 1C; protein tyrosine phosphatase; non-receptor type 6

other names :

tyrosine-protein phosphatase non-receptor type 6; Tyrosine-protein phosphatase non-receptor type 6; tyrosine-protein phosphatase non-receptor type 6; SH2 phosphatase 1; protein-tyrosine phosphatase SHP-1; protein tyrosine phosphatase, non-receptor type 6; Protein-tyrosine phosphatase SHP-1

products gene name :

PTPN6

other gene names :

Ptpn6; Ptpn6; Ptph6; Shp-1; Ptph6

uniprot entry name :

PTN6_RAT

reactivity :

Rat

sequence length :

613

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat PTPN6. No significant cross-reactivity or interference between Rat PTPN6 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 46.88 pg/ml-3000 pg/ml. Sensitivity: 11.72 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PTPN6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTPN6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPN6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPN6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

16758788

ncbi acc num :

NP_446360.1

ncbi gb acc num :

NM_053908.1

uniprot acc num :

P81718

ncbi mol weight :

69,578 Da

ncbi pathways :

Adherens Junction Pathway (83462); Adherens Junction Pathway (481); B Cell Receptor Signaling Pathway (198486); B Cell Receptor Signaling Pathway (83473); B Cell Receptor Signaling Pathway (492); EGFR1 Signaling Pathway (198427); IL-2 Signaling Pathway (198476); IL-3 Signaling Pathway (198504); IL-4 Signaling Pathway (198434); IL-5 Signaling Pathway (198483)

ncbi summary :

plays a role in activation and proliferation of glial cells and astrocytes [RGD, Feb 2006]

uniprot summary :

SHP-1: an SH2-containing ubiquitously expressed tyrosine-specific protein phosphatase. Plays a key role in hematopoiesis. Directly links growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Three alternatively spliced isoforms have been described. Protein type: EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis. Cellular Component: cytoplasm; nucleolus; alpha-beta T cell receptor complex; intracellular; intercellular junction; nucleus. Molecular Function: phosphotyrosine binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; SH2 domain binding; SH3 domain binding; protein kinase binding; natural killer cell lectin-like receptor binding. Biological Process: negative regulation of MAP kinase activity; negative regulation of peptidyl-tyrosine phosphorylation; peptidyl-tyrosine phosphorylation; negative regulation of MAPKKK cascade; natural killer cell mediated cytotoxicity; positive regulation of cell adhesion mediated by integrin; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation; regulation of release of sequestered calcium ion into cytosol; positive regulation of phosphoinositide 3-kinase cascade; negative regulation of humoral immune response mediated by circulating immunoglobulin; cell proliferation; B cell receptor signaling pathway; negative regulation of T cell proliferation; negative regulation of B cell receptor signaling pathway; regulation of B cell differentiation; positive regulation of cell proliferation; response to wounding; hemopoietic progenitor cell differentiation; abortive mitotic cell cycle; platelet formation; cell differentiation

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage