ELISA/assay

Human Rho-associated protein kinase 1, ROCK1 ELISA Kit

MBS9318720

96 Strip Wells

600 USD

ELISA Kit

Human Rho-associated protein kinase 1, ROCK1 ELISA Kit

Rho-associated, coiled-coil containing protein kinase 1

Human Rho-associated protein kinase 1 (ROCK1) ELISA kit; MGC131603; MGC43611; P160ROCK; PRO0435; Rho kinase; p160-ROCK; renal carcinoma antigen NY-REN-35; Rho-associated; coiled-coil containing protein kinase 1

rho-associated protein kinase 1; Rho-associated protein kinase 1; rho-associated protein kinase 1; p160-ROCK; Rho kinase; p160 ROCK-1; renal carcinoma antigen NY-REN-35; rho-associated, coiled-coil-containing protein kinase 1; rho-associated, coiled-coil-containing protein kinase I; Rho-associated, coiled-coil containing protein kinase 1; Renal carcinoma antigen NY-REN-35; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK

ROCK1

ROCK1; ROCK1; ROCK-I; P160ROCK; ROCK-I; p160ROCK

ROCK1_HUMAN

Human

No significant cross-reactivity or interference between Human ROCK1 and analogues was observed.

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ROCK1 concentrations in Human serum, plasma and other body fluids. Using Purified Human ROCK1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ROCK1 and ROCK1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ROCK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Detection Range: The detection range of this kit is 0.25ng/ml-8ng/ml.

Sensitivity: The sensitivity of this kit is 0.1ng/ml. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ROCK1 concentrations in Human serum, plasma and other body fluids. Using Purified Human ROCK1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ROCK1 and ROCK1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ROCK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4885583

NP_005397.1

NM_005406.2

Q13464

158,175 Da

Apoptosis Pathway 105648!!Apoptotic Cleavage Of Cellular Proteins Pathway 105678!!Apoptotic Execution Phase Pathway 105677!!Axon Guidance Pathway 83065!!Axon Guidance Pathway 476!!Axon Guidance Pathway 105688!!Chemokine Signaling Pathway 99051!!Chemokine Signaling Pathway 96864!!Developmental Biology Pathway 477129!!EPHA Forward Signaling Pathway 138041

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq, Jul 2008]

Function: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.14 PublicationsManual assertion based on experiment in:Ref.1. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Enzyme regulation: Activated by RHOA binding. Inhibited by Y-27632.

96 Strip Wells

600 USD