ELISA/assay

Human Pepsinogen I ELISA Kit

MBS931871

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human Pepsinogen I ELISA Kit

[Pepsinogen I]

[Human Pepsinogen I ELISA Kit; Pepsinogen I]

Human

This assay has high sensitivity and excellent specificity for detection of human pepsinogen I. No significant cross-reactivity or interference between human pepsinogen I and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 6.25 ng/ml -400 ng/ml. Sensitivity: 1.56 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for pepsinogen I has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any pepsinogen I present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for pepsinogen I is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of pepsinogen I bound in the initial step. The color development is stopped and the intensity of the color is measured.

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145