ELISA/assay

Human Eukaryotic translation initiation factor 4 gamma 1, EIF4G1 ELISA Kit

MBS9318670

48-Strip-Wells

470 USD

ELISA Kit

Human Eukaryotic translation initiation factor 4 gamma 1, EIF4G1 ELISA Kit

[eukaryotic translation initiation factor 4 gamma, 1]

[Human Eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) ELISA kit; DKFZp686A1451; EIF4F; EIF4G; p220; EIF4-gamma; eukaryotic translation initiation factor 4 gamma; 1]

[eukaryotic translation initiation factor 4 gamma 1 isoform 4; Eukaryotic translation initiation factor 4 gamma 1; eukaryotic translation initiation factor 4 gamma 1; EIF4-gamma; eIF-4-gamma 1; eucaryotic translation initiation factor 4G; eukaryotic translation initiation factor 4 gamma, 1; p220]

[EIF4G1]

[EIF4G1; EIF4G1; P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1; EIF4F; EIF4G; EIF4GI; eIF-4-gamma 1; eIF-4G 1; eIF-4G1]

IF4G1_HUMAN

Human

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Body fluids, tissue homogenates, secretions or feces samples. Assay Type: Quantitative Sandwich. Detection Range: 0.5 ng/ml - 16 ng/ml. Sensitivity: 0.1 ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of EIF4G1 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

302699239

NP_004944.3

NM_004953.4

Q04637

175,491 Da

Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway (105970); Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); Cap-dependent Translation Initiation Pathway (105967); Cytokine Signaling In Immune System Pathway (366171); Deadenylation Of MRNA Pathway (160994); Deadenylation-dependent MRNA Decay Pathway (160993); Destabilization Of MRNA By AUF1 (hnRNP D0) Pathway (187212); Eukaryotic Translation Initiation Pathway (105966); GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway (105973); Gene Expression Pathway (105937)

The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010]

eIF4G: Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5 -terminal secondary structure and recruitment of mRNA to the ribosome. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with CIRBP and MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis. Interacts with RBM4. Belongs to the eIF4G family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: RNA-binding; Translation initiation; Translation. Chromosomal Location of Human Ortholog: 3q27.1. Cellular Component: membrane; eukaryotic translation initiation factor 4F complex; cytosol. Molecular Function: protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity. Biological Process: poly(A) tail shortening; cellular protein metabolic process; viral reproduction; translation; cytokine and chemokine mediated signaling pathway; insulin receptor signaling pathway; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; regulation of translational initiation; mRNA catabolic process, deadenylation-dependent decay

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095