Human Eyes absent homolog 1, EYA1 ELISA Kit | MyBioSource MBS9317898 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Eyes absent homolog 1, EYA1 ELISA Kit

catalog :

MBS9317898

quantity :

96 Strip Wells

price :

600 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS9317898

products type :

ELISA Kit

products full name :

Human Eyes absent homolog 1, EYA1 ELISA Kit

products short name :

eyes absent homolog 1 (Drosophila)

products name syn :

Human Eyes absent homolog 1 (EYA1) ELISA kit; BOP; BOR; MGC141875; OTTHUMP00000195055; eyes absent 1; eyes absent homolog 1 (Drosophila)

other names :

eyes absent homolog 1 isoform 1; Eyes absent homolog 1; eyes absent homolog 1; EYA transcriptional coactivator and phosphatase 1

products gene name :

EYA1

other gene names :

EYA1; EYA1; BOP; BOR; BOS1; OFC1

uniprot entry name :

EYA1_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human EYA1 and analogues was observed.

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated EYA1 concentrations in Human serum, plasma and other body fluids. Using Purified Human EYA1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EYA1 and EYA1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EYA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Detection Range: The detection range of this kit is 62.5 pg/ml - 2000 pg/ml.

other info2 :

Sensitivity: The sensitivity of this kit is 10 pg/ml. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated EYA1 concentrations in Human serum, plasma and other body fluids. Using Purified Human EYA1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EYA1 and EYA1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EYA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

19923100

ncbi acc num :

NP_000494.2

ncbi gb acc num :

NM_000503.5

uniprot acc num :

Q99502

ncbi mol weight :

64,593 Da

ncbi pathways :

Transcriptional Misregulation In Cancer Pathway 523016!!Transcriptional Misregulation In Cancer Pathway 522987

ncbi summary :

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may play a role in the developing kidney, branchial arches, eye, and ear. Mutations of this gene have been associated with branchiootorenal dysplasia syndrome, branchiootic syndrome, and sporadic cases of congenital cataracts and ocular anterior segment anomalies. A similar protein in mice can act as a transcriptional activator. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Dec 2013]

uniprot summary :

Function: Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Has also phosphatase activity with proteins phosphorylated on Ser and Thr residues (in vitro). Required for normal embryonic development of the craniofacial and trunk skeleton, kidneys and ears. Together with SIX1, it plays an important role in hypaxial muscle development; in this it is functionally redundant with EYA2.1 PublicationManual assertion based on experiment in:Ref.12. Catalytic activity: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 PublicationManual assertion based on experiment in:Ref.12. Cofactor: Binds 1 Mg2+ ion per subunit

size :

96 Strip Wells

price :

600 USD

more info or order :

MyBioSource product webpage