ELISA/assay

Human Serine/threonine-protein kinase ULK1, ULK1 ELISA Kit

MBS9317785

48-Strip-Wells

435 USD

ELISA Kit

Human Serine/threonine-protein kinase ULK1, ULK1 ELISA Kit

unc-51-like kinase 1 (C. elegans)

Human Serine/threonine-protein kinase ULK1 (ULK1) ELISA kit; ATG1; FLJ38455; FLJ46475; UNC51; Unc51.1; ATG1 autophagy related 1 homolog; Unc-51-like kinase 1; unc-51-like kinase 1 (C. elegans)

serine/threonine-protein kinase ULK1; Serine/threonine-protein kinase ULK1; serine/threonine-protein kinase ULK1; ATG1 autophagy related 1 homolog; autophagy-related protein 1 homolog; unc-51 like autophagy activating kinase 1; Autophagy-related protein 1 homolog; ATG1; hATG1; Unc-51-like kinase 1

ULK1

ULK1; ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1; KIAA0722; ATG1; hATG1

ULK1_HUMAN

Human

No significant cross-reactivity or interference between Human ULK1 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 31.2pg/ml-1000pg/ml. Sensitivity: 5.0pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ULK1 concentrations in Human serum, plasma and other body fluids. Using Purified Human ULK1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ULK1 and ULK1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ULK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ULK1 concentrations in Human serum, plasma and other body fluids. Using Purified Human ULK1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ULK1 and ULK1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ULK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4507831

NP_003556.1

NM_003565.2

O75385

112,631 Da

Regulation Of Autophagy Pathway (83058); Regulation Of Autophagy Pathway (468); Senescence And Autophagy Pathway (198780); MTOR Signaling Pathway (83059); MTOR Signaling Pathway (469)

ULK1: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. Interacts with GABARAP and GABARAPL2. Interacts (via C- terminus) with ATG13/KIAA0652. Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation. Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. Protein type: EC 2.7.11.1; Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Autophagy; Other group; ULK family. Chromosomal Location of Human Ortholog: 12q24.3. Cellular Component: neuron projection; cytoplasmic vesicle membrane; cell soma; pre-autophagosomal structure membrane; cytoplasm; autophagic vacuole; cytosol. Molecular Function: protein serine/threonine kinase activity; protein binding; protein complex binding; protein kinase binding; ATP binding; Rab GTPase binding. Biological Process: axon extension; protein amino acid autophosphorylation; neurite regeneration; negative regulation of collateral sprouting; cerebellar granule cell differentiation; protein amino acid phosphorylation; radial glia guided migration of granule cell; response to starvation; protein localization; cellular response to nutrient levels; receptor internalization; Ras protein signal transduction; regulation of autophagy; regulation of nerve growth factor receptor signaling pathway; positive regulation of macroautophagy; neurite development; positive regulation of autophagy; autophagic vacuole formation

48-Strip-Wells

435 USD

96-Strip-Wells

600