ELISA/assay

Human Cyclin-G-associated kinase, GAK ELISA Kit

MBS9317726

48-Strip-Wells

470 USD

ELISA Kit

Human Cyclin-G-associated kinase, GAK ELISA Kit

[cyclin G associated kinase]

[Human Cyclin-G-associated kinase (GAK) ELISA kit; FLJ16629; FLJ40395; MGC99654; ; cyclin G associated kinase]

[cyclin-G-associated kinase isoform 2; Cyclin-G-associated kinase; cyclin-G-associated kinase; auxilin-2; cyclin G associated kinase]

[GAK]

[GAK; GAK; DNAJ26; DNAJC26]

GAK_HUMAN

Human

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Body fluids, tissue homogenates, secretions or feces samples. Assay Type: Quantitative Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of GAK (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

558472758

NP_001273762.1

NM_001286833.1

O14976

143,191 Da

Clathrin Derived Vesicle Budding Pathway (119545); Golgi Associated Vesicle Biogenesis Pathway (119546); Membrane Trafficking Pathway (106160); Trans-Golgi Network Vesicle Budding Pathway (106165)

In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

GAK: Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Cell cycle regulation; Protein kinase, Other; Other group; NAK family. Chromosomal Location of Human Ortholog: 4p16. Cellular Component: Golgi apparatus; focal adhesion; intracellular membrane-bound organelle; membrane; perinuclear region of cytoplasm. Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding. Biological Process: endoplasmic reticulum organization and biogenesis; epidermal cell differentiation; cell cycle; forebrain morphogenesis; protein amino acid phosphorylation; Golgi organization and biogenesis; cell development

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095