Human Lysyl oxidase homolog 1, LOXL1 ELISA Kit | MyBioSource MBS931742 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Lysyl oxidase homolog 1, LOXL1 ELISA Kit

catalog :

MBS931742

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS931742

products type :

ELISA Kit

products full name :

Human Lysyl oxidase homolog 1, LOXL1 ELISA Kit

products short name :

lysyl oxidase-like 1

products name syn :

Human Lysyl oxidase homolog 1 (LOXL1) ELISA kit; LOL; LOXL; lysyl oxidase homolog 1; lysyl oxidase-like 1

other names :

lysyl oxidase homolog 1 preproprotein; Lysyl oxidase homolog 1; lysyl oxidase homolog 1; lysyl oxidase-like protein 1; lysyl oxidase-like 1; Lysyl oxidase-like protein 1; LOL

products gene name :

LOXL1

other gene names :

LOXL1; LOXL1; LOL; LOXL; LOXL; LOL

uniprot entry name :

LOXL1_HUMAN

reactivity :

Human

sequence length :

574

specificity :

This assay has high sensitivity and excellent specificity for detection of human LOXL1. No significant cross-reactivity or interference between human LOXL1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human LOXL1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LOXL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LOXL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LOXL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LOXL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

67782346

ncbi acc num :

NP_005567.2

ncbi gb acc num :

NM_005576.2

uniprot acc num :

Q08397

ncbi mol weight :

63,110 Da

ncbi pathways :

Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Formation Pathway (645288); Crosslinking Of Collagen Fibrils Pathway (730308); Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]

uniprot summary :

LOXL1: Active on elastin and collagen substrates. Genetic variations in LOXL1 are a cause of susceptibility to exfoliation syndrome (XFS); also called exfoliation glaucoma (XFG). XFS is a disorder characterized by accumulation of abnormal fibrillar deposits in the anterior segment of the eye. In addition to being a cause of glaucoma and glaucomatous optic neuropathy, exfoliation syndrome has also been associated with lens zonule weakness, cataract formation, and systemic vascular complications due to deposition of exfoliation material in extraocular tissues. Susceptibility to exfoliation syndrome is conferred by a risk haplotype that includes two LOXL1 coding non-synonymous SNPs (Arg141Leu and Gly153Asp) and one intronic SNP. Arg141Leu and Gly153Asp are sufficient to confer disease susceptibility in some populations. Belongs to the lysyl oxidase family. Protein type: EC 1.4.3.-; Secreted, signal peptide; Oxidoreductase; Extracellular matrix; Secreted. Chromosomal Location of Human Ortholog: 15q22. Cellular Component: extracellular matrix; extracellular space; acrosome; extracellular region; basement membrane. Molecular Function: copper ion binding; oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor. Biological Process: extracellular matrix organization and biogenesis; response to lipopolysaccharide; protein amino acid deamination. Disease: Exfoliation Syndrome

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage