ELISA/assay

Human Krueppel-like factor 2, KLF2 ELISA Kit

MBS931735

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human Krueppel-like factor 2, KLF2 ELISA Kit

[Kruppel-like factor 2 (lung)]

[Human Krueppel-like factor 2 (KLF2) ELISA kit; LKLF; Kruppel-like factor; Kruppel-like factor LKLF; lung Kruppel-like zinc finger transcription factor; Kruppel-like factor 2 (lung)]

[Krueppel-like factor 2; Krueppel-like factor 2; Krueppel-like factor 2; Kruppel-like factor LKLF; lung krueppel-like factor; Kruppel-like factor 2 (lung); lung Kruppel-like zinc finger transcription factor; Kruppel-like factor 2; Lung krueppel-like factor]

[KLF2]

[KLF2; KLF2; LKLF; LKLF]

KLF2_HUMAN

Human

355

This assay has high sensitivity and excellent specificity for detection of human Kim-1. No significant cross-reactivity or interference between human Kim-1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Urine, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.043 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Kim-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Kim-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Kim-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Kim-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

7706469

NP_057354.1

NM_016270.2

Q9Y5W3

37,420 Da

FoxO Signaling Pathway (921162)

Kruppel-like factors (KLFs) are a family of broadly expressed zinc finger transcription factors. KLF2 regulates T-cell trafficking by promoting expression of the lipid-binding receptor S1P1 (S1PR1; MIM 601974) and the selectin CD62L (SELL; MIM 153240) (summary by Weinreich et al., 2009 [PubMed 19592277]).[supplied by OMIM, Feb 2011]

KLF2: Binds to the CACCC box in the beta-globin gene promoter and activates transcription. Belongs to the krueppel C2H2-type zinc-finger protein family. Protein type: DNA-binding; C2H2-type zinc finger protein. Chromosomal Location of Human Ortholog: 19p13.11. Cellular Component: nucleus. Molecular Function: protein binding; sequence-specific DNA binding; metal ion binding; transcription factor activity. Biological Process: erythrocyte maturation; transcription, DNA-dependent; in utero embryonic development; multicellular organism growth; negative regulation of interleukin-6 production; cell morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein metabolic process; regulation of gene expression, epigenetic

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145