ELISA/assay

Human Tripartite motif-containing protein 35, TRIM35 ELISA Kit

MBS9317166

48-Strip-Wells

435 USD

ELISA Kit

Human Tripartite motif-containing protein 35, TRIM35 ELISA Kit

tripartite motif-containing 35

Human Tripartite motif-containing protein 35 (TRIM35) ELISA kit; HLS5; KIAA1098; MAIR; MGC17233; ; tripartite motif-containing 35

tripartite motif-containing protein 35; Tripartite motif-containing protein 35; tripartite motif-containing protein 35; tripartite motif-containing 35; hemopoietic lineage switch protein 5; tripartite motif containing 35; Hemopoietic lineage switch protein 5

TRIM35

TRIM35; TRIM35; HLS5; MAIR; HLS5; KIAA1098

TRI35_HUMAN

Human

No significant cross-reactivity or interference between Human TRIM35 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TRIM35 concentrations in Human serum, plasma and other body fluids. Using Purified Human TRIM35 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TRIM35 and TRIM35 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TRIM35 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TRIM35 concentrations in Human serum, plasma and other body fluids. Using Purified Human TRIM35 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TRIM35 and TRIM35 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TRIM35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

94536782

NP_741983.2

NM_171982.3

Q9UPQ4

56,540 Da

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified. [provided by RefSeq, Jul 2008]

TRIM35: May play a role as a tumor suppressor. Implicated in the cell death mechanism. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 8p21.2. Cellular Component: nucleoplasm; cytoplasm; nucleus. Molecular Function: zinc ion binding. Biological Process: negative regulation of cell proliferation; apoptosis; positive regulation of apoptosis; innate immune response; negative regulation of mitotic cell cycle

48-Strip-Wells

435 USD

96-Strip-Wells

600