Human CD81 antigen, CD81 ELISA Kit | MyBioSource MBS931608 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human CD81 antigen, CD81 ELISA Kit

catalog :

MBS931608

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS931608

products type :

ELISA Kit

products full name :

Human CD81 antigen, CD81 ELISA Kit

products short name :

CD81 molecule

products name syn :

Human CD81 antigen (CD81) ELISA kit; S5.7; TAPA1; TSPAN28; 26 kDa cell surface protein TAPA-1; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1) ; target of antiproliferative antibody 1; CD81 molecule

other names :

CD81 antigen isoform 1; CD81 antigen; CD81 antigen; tspan-28; tetraspanin-28; 26 kDa cell surface protein TAPA-1; CD81 antigen (target of antiproliferative antibody 1); CD81 molecule; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28; Tspan-28; CD_antigen: CD81

products gene name :

CD81

other gene names :

CD81; CD81; S5.7; CVID6; TAPA1; TSPAN28; TAPA1; TSPAN28; Tspan-28

uniprot entry name :

CD81_HUMAN

reactivity :

Human

sequence length :

236

specificity :

This assay has high sensitivity and excellent specificity for detection of human CD81. No significant cross-reactivity or interference between human CD81 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: Typically less than 0.039 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD81 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD81 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD81 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD81 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4757944

ncbi acc num :

NP_004347.1

ncbi gb acc num :

NM_004356.3

uniprot acc num :

P60033

ncbi mol weight :

25,809 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); B Cell Receptor Signaling Pathway (198909); B Cell Receptor Signaling Pathway (83081); B Cell Receptor Signaling Pathway (492); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413); Malaria Pathway (152665); Malaria Pathway (152657)

ncbi summary :

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

uniprot summary :

CD81: May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV. Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6); also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the tetraspanin (TM4SF) family. Protein type: Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; immunological synapse; vesicle. Molecular Function: protein binding. Biological Process: cell proliferation; virion attachment, binding of host cell surface receptor; protein localization; positive regulation of peptidyl-tyrosine phosphorylation; entry of virus into host cell; regulation of immune response; activation of MAPK activity; positive regulation of cell proliferation; positive regulation of 1-phosphatidylinositol 4-kinase activity; positive regulation of B cell proliferation; phosphatidylinositol biosynthetic process; phosphatidylinositol metabolic process. Disease: Immunodeficiency, Common Variable, 6

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage