ELISA/assay

Human Creatine Kinase MM isoenzyme, CK-MM ELISA Kit

MBS931554

48-Strip-Wells

565 USD

ELISA Kit

Human Creatine Kinase MM isoenzyme, CK-MM ELISA Kit

Creatine Kinase MM isoenzyme (CK-MM)

Human Creatine Kinase MM isoenzyme (CK-MM) ELISA Kit; Creatine Kinase MM isoenzyme (CK-MM)

creatine kinase, CK-MM {N-terminal} {EC 2.7.3.2}, partial

CK-MM

Human

20

This assay has high sensitivity and excellent specificity for detection of human CK-MM. No significant cross-reactivity or interference between human CK-MM and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 mU/ml -200 mU/ml. Sensitivity: The minimum detectable dose of human CK-MM is typically less than 0.78 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CK-MM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CK-MM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CK-MM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CK-MM bound in the initial step. The color development is stopped and the intensity of the color is measured.

260938

AAB24342.1

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48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150