ELISA/assay

Human Histone-lysine N-methyltransferase EZH2, EZH2 ELISA Kit

MBS931552

48-Strip-Wells

510 USD

ELISA Kit

Human Histone-lysine N-methyltransferase EZH2, EZH2 ELISA Kit

enhancer of zeste homolog 2 (Drosophila)

Human Histone-lysine N-methyltransferase EZH2 (EZH2) ELISA kit; ENX-1; ENX1; EZH1; KMT6; MGC9169; enhancer of zeste 2; lysine N-methyltransferase 6; enhancer of zeste homolog 2 (Drosophila)

histone-lysine N-methyltransferase EZH2 isoform c; Histone-lysine N-methyltransferase EZH2; histone-lysine N-methyltransferase EZH2; enhancer of zeste homolog 2; lysine N-methyltransferase 6; enhancer of zeste 2 polycomb repressive complex 2 subunit; ENX-1; Enhancer of zeste homolog 2; Lysine N-methyltransferase 6

EZH2

EZH2; EZH2; WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A; KMT6

EZH2_HUMAN

Human

746

This assay has high sensitivity and excellent specificity for detection of Human EZH2. No significant cross-reactivity or interference between Human EZH2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

322506097

NP_001190176.1

NM_001203247.1

Q15910

85,363 Da

Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Epigenetic Regulation Of Gene Expression Pathway (980449); Gene Expression Pathway (105937); Integrated Pancreatic Cancer Pathway (711360); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); Oxidative Stress Induced Senescence Pathway (905993); PRC2 Methylates Histones And DNA Pathway (980450); MiRs In Muscle Cell Differentiation Pathway (672449)

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]

EZH2: a histone H3 Lys 27 (H3K27) methyltransferase and Polycomb group protein with oncogenic activity. A master regulatory protein that plays a critical role in development. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates K9 and K27 of histone H3, leading to transcriptional repression of the affected target genes. PRC2 includes the Ezh2, EED, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The recruitment of PRC2 to a promoter leads to increased levels of di- and trimethylation of H3K27 (H3K27me2/3), regulating the balance between self-renewal and differentiation of embryonic stem cells (ESCs). The recruitment of PRC2 to promoters in ES cells is interdependent on Jarid 2. ESCs lacking the PRC2 component EED are deficient in Jarid2 promoter activity, while the knockdown of Jarid 2 reduces PRC2 at its target promoters. Able to mono-, di- and trimethylate K27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, p14ARF and retinoic acid target genes. Binds ATRX via the SET domain. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC1 and HDAC2. Interacts with PRAME. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. Expression decreases during senescence of embryonic fibroblasts. Expression peaks at the G1/S phase boundary. Expression is induced by E2F1, E2F2 and E2F3. Expression is reduced in cells subject to numerous types of stress including UV-, IR- and bleomycin-induced DNA damage and by activation of p53. It has been reported that phosphorylation by AKT1 on S21 reduces methyltransferase activity. Protein type: Methyltransferase; Methyltransferase, protein lysine; EC 2.1.1.43. Chromosomal Location of Human Ortholog: 7q35-q36. Cellular Component: nucleoplasm; pronucleus; ESC/E(Z) complex; cytoplasm; nuclear chromatin; nucleus. Molecular Function: histone methyltransferase activity; protein binding; DNA binding; sequence-specific DNA binding; chromatin DNA binding; RNA binding; protein-lysine N-methyltransferase activity; chromatin binding; histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific). Biological Process: negative regulation of striated muscle cell differentiation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of gliogenesis; rhythmic process; negative regulation of transcription from RNA polymerase II promoter; satellite cell compartment self-renewal involved in skeletal muscle regeneration; regulation of circadian rhythm; regulation of gene expression, epigenetic; regulation of cell proliferation; negative regulation of retinoic acid receptor signaling pathway; positive regulation of MAP kinase activity; cerebellar cortex development; regulation of transcription, DNA-dependent; negative regulation of gene expression, epigenetic; negative regulation of epidermal cell differentiation; DNA methylation; gene expression; negative regulation of transcription, DNA-dependent. Disease: Weaver Syndrome

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800