ELISA/assay

Mouse Ephrin type-A receptor 2, EPHA2 ELISA Kit

MBS931518

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Mouse Ephrin type-A receptor 2, EPHA2 ELISA Kit

[EPH receptor A2]

[Mouse Ephrin type-A receptor 2 (EPHA2) ELISA kit; ARCC2; ECK; ephrin receptor EphA2; epithelial cell receptor protein tyrosine kinase; protein tyrosine kinase; soluble EPHA2 variant 1; EPH receptor A2]

[ephrin type-A receptor 2; Ephrin type-A receptor 2; ephrin type-A receptor 2; epithelial cell kinase; tyrosine-protein kinase receptor ECK; tyrosine-protein kinase receptor MPK-5; tyrosine-protein kinase receptor SEK-2; Eph receptor A2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; Tyrosine-protein kinase receptor MPK-5; Tyrosine-protein kinase receptor SEK-2]

[EPHA2]

[Epha2; Epha2; Eck; Myk2; Sek2; Sek-2; AW545284; Eck; Myk2; Sek2]

EPHA2_MOUSE

Mouse

977

This assay has high sensitivity and excellent specificity for detection of human ENOX2. No significant cross-reactivity or interference between human ENOX2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates. Assay Type: Quantitative Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.81 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ENOX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ENOX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ENOX2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ENOX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

32484983

NP_034269.2

NM_010139.3

Q03145

108,852 Da

Axon Guidance Pathway (83262); Axon Guidance Pathway (476); Axon Guidance Pathway (970585); Developmental Biology Pathway (970813); EPH-Ephrin Signaling Pathway (1000783); EPH-ephrin Mediated Repulsion Of Cells Pathway (1000787); EPHA-mediated Growth Cone Collapse Pathway (1000784); PI3K-Akt Signaling Pathway (692242); PI3K-Akt Signaling Pathway (692979); Rap1 Signaling Pathway (869018)

EphA2: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 AND -A5. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Overexpressed in many cancers including aggressive ovarian, cervical and breast carcinomas, and lung cancer. Expression correlates with degree of angiogenesis, metastasis and xenograft tumor growth. Soluble receptor inhibits tumor growth and angiogenesis in mice. Protein type: Protein kinase, TK; Membrane protein, integral; EC 2.7.10.1; Kinase, protein; Protein kinase, tyrosine (receptor); TK group; Eph family. Cellular Component: cell projection; focal adhesion; cell surface; membrane; integral to plasma membrane; integral to membrane; plasma membrane; intracellular; cell junction. Molecular Function: transferase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; ephrin receptor activity; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity. Biological Process: neural tube development; peptidyl-tyrosine phosphorylation; apoptosis; notochord formation; osteoclast differentiation; bone remodeling; regulation of blood vessel endothelial cell migration; protein amino acid phosphorylation; neuron differentiation; notochord morphogenesis; regulation of cell adhesion mediated by integrin; ephrin receptor signaling pathway; protein kinase B signaling cascade; angiogenesis; cell adhesion; cell differentiation; vasculogenesis; skeletal development; axial mesoderm formation; blood vessel development; cell migration; negative regulation of protein kinase B signaling cascade; mammary gland epithelial cell proliferation; regulation of angiogenesis; keratinocyte differentiation; osteoblast differentiation; DNA damage response, signal transduction resulting in induction of apoptosis; notochord cell development; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145