ELISA/assay

Human Septin-2, SEPT2 ELISA Kit

MBS931487

48-Strip-Wells

510 USD

ELISA Kit

Human Septin-2, SEPT2 ELISA Kit

septin 2

Human Septin-2 (SEPT2) ELISA kit; DIFF6; KIAA0158; NEDD5; Pnutl3; hNedd5; OTTHUMP00000200251; neural precursor cell expressed; developmentally down-regulated 5; septin 2

septin-2 isoform a; Septin-2; septin-2; neural precursor cell expressed, developmentally down-regulated 5; neural precursor cell expressed developmentally down-regulated protein 5; septin 2; Neural precursor cell expressed developmentally down-regulated protein 5; NEDD-5

SEPT2; SEPT2; DIFF6; NEDD5; NEDD-5; Pnutl3; hNedd5; DIFF6; KIAA0158; NEDD5; NEDD-5

SEPT2_HUMAN

Human

361

This assay has high sensitivity and excellent specificity for detection of human SEPT2. No significant cross-reactivity or interference between human SEPT2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml -1600 pg/ml. Sensitivity: The minimum detectable dose of human SEPT2 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEPT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEPT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEPT2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEPT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

56549636

NP_001008491.1

NM_001008491.2

Q15019

41,487 Da

Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); CDC42 Signaling Events Pathway (137994)

SEPT2: a member of the septin family of proteins. May be involved in the process of cytokinesis. Accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody. In interphase and postmitotic cells, localized to fibrous or granular structures, depending on the growth state of the cell. Protein type: Motility/polarity/chemotaxis; Cell cycle regulation; Cytoskeletal; Cell development/differentiation; Hydrolase. Chromosomal Location of Human Ortholog: 2q37. Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytoplasm; nucleolus; exocyst; spindle; synapse; midbody; cell cortex; nucleus; cleavage furrow; actin cytoskeleton. Molecular Function: protein binding; GTP binding; protein complex scaffold; enzyme regulator activity. Biological Process: mitosis; smoothened signaling pathway; regulation of protein localization; cell division; regulation of catalytic activity; cilium biogenesis; regulation of L-glutamate transport; neurite development

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800