Human Fragile X mental retardation 1 protein, FMR1 ELISA Kit | MyBioSource MBS931448 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Fragile X mental retardation 1 protein, FMR1 ELISA Kit

catalog :

MBS931448

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS931448

products type :

ELISA Kit

products full name :

Human Fragile X mental retardation 1 protein, FMR1 ELISA Kit

products short name :

fragile X mental retardation 1

products name syn :

Human Fragile X mental retardation 1 protein (FMR1) ELISA kit; FMRP; FRAXA; MGC87458; POF; POF1; premature ovarian failure 1; fragile X mental retardation 1

other names :

fragile X mental retardation protein 1 isoform ISO6; Fragile X mental retardation protein 1; fragile X mental retardation protein 1; fragile X mental retardation 1

products gene name :

FMR1

other gene names :

FMR1; FMR1; POF; FMRP; POF1; FRAXA; FMRP; Protein FMR-1

uniprot entry name :

FMR1_HUMAN

reactivity :

Human

sequence length :

537

specificity :

This assay has high sensitivity and excellent specificity for detection of human FMR1. No significant cross-reactivity or interference between human FMR1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of human FMR1 is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FMR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FMR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FMR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FMR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

297374777

ncbi acc num :

NP_001172004.1

ncbi gb acc num :

NM_001185075.1

uniprot acc num :

Q06787

ncbi mol weight :

71,174 Da

ncbi pathways :

RNA Transport Pathway (177876); RNA Transport Pathway (175229)

ncbi summary :

The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene. [provided by RefSeq, May 2010]

uniprot summary :

FMR1: Translation repressor. Component of the CYFIP1-EIF4E- FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression. RNA- binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C). Component of the CYFIP1-EIF4E-FMR1 complex which is composed of CYFIP, EIF4E and FMR1. Interacts with CYFIP1 and CYFIP2. The interaction with brain cytoplasmic RNA 1 (BC1) increases binding affinity for the CYFIP1-EIF4E complex in the brain. Homooligomer. Found in a RNP granule complex with IGF2BP1. Directly interacts with SMN and TDRD3. Interacts with the SMN core complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts with FXR1, FXR2, IGF2BP1, NUFIP1, NUFIP2, MCRS1 and RANBP9. Highest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells. Belongs to the FMR1 family. 8 isoforms of the human protein are produced by alternative splicing. Protein type: Nucleolus; RNA-binding; Translation. Chromosomal Location of Human Ortholog: Xq27.3. Cellular Component: nucleoplasm; mRNA cap complex; polysomal ribosome; microtubule; membrane; stress granule; cytoplasm; nucleolus; dendritic spine; synapse; dendritic shaft. Molecular Function: mRNA binding; protein binding; RNA binding; microtubule binding. Biological Process: negative regulation of translational initiation; mRNA transport; central nervous system development; negative regulation of translation. Disease: Fragile X Mental Retardation Syndrome; Fragile X Tremor/ataxia Syndrome; Premature Ovarian Failure 1

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage