ELISA/assay

Mouse Ferritin heavy chain, FTH1 ELISA Kit

MBS931412

48-Strip-Wells

510 USD

ELISA Kit

Mouse Ferritin heavy chain, FTH1 ELISA Kit

ferritin, heavy polypeptide 1

Mouse Ferritin heavy chain (FTH1) ELISA kit; FHC; FTH; FTHL6; MGC104426; PIG15; PLIF; apoferritin; placenta immunoregulatory factor; proliferation-inducing protein 15; ferritin; heavy polypeptide 1

ferritin heavy chain; Ferritin heavy chain; ferritin heavy chain; H-ferritin; ferritin H subunit; ferritin heavy chain 1

FTH1

Fth1; Fth1; FHC; Fth; HFt; MFH; Fth; Ferritin H subunit

FRIH_MOUSE

Mouse

182

This assay has high sensitivity and excellent specificity for detection of mouse FTH1. No significant cross-reactivity or interference between mouse FTH1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: 0.078 ng /ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FTH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FTH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FTH1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FTH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

6753912

NP_034369.1

NM_010239.2

P09528

21,067 Da

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (1000591); Clathrin Derived Vesicle Budding Pathway (1000294); Golgi Associated Vesicle Biogenesis Pathway (1000295); Iron Homeostasis Pathway (198312); Iron Uptake And Transport Pathway (1001366); Membrane Trafficking Pathway (1000288); Mineral Absorption Pathway (212238); Mineral Absorption Pathway (212220); Scavenging By Class A Receptors Pathway (1000593); Transmembrane Transport Of Small Molecules Pathway (970706)

Ferritin-H: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Belongs to the ferritin family. Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.16.3.1. Cellular Component: mitochondrion; nucleus. Molecular Function: ferroxidase activity; ferric iron binding; metal ion binding; oxidoreductase activity. Biological Process: cellular iron ion homeostasis; negative regulation of fibroblast proliferation; iron ion transport; intracellular sequestering of iron ion

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800