catalog number :
MBS9312505
products type :
ELISA Kit
products full name :
Human Alkaline Ceramidase 2 (ACER2) ELISA Kit
products short name :
[Alkaline Ceramidase 2 (ACER2)]
other names :
[alkaline ceramidase 2; Alkaline ceramidase 2; alkaline ceramidase 2; haCER2; alkCDase 2; alkaline CDase 2; ceramide hydrolase; alkaline ceramidase 2; Acylsphingosine deacylase 3-like; N-acylsphingosine amidohydrolase 3-like]
products gene name :
[ACER2]
other gene names :
[ACER2; ACER2; ASAH3L; ALKCDase2; ASAH3L; AlkCDase 2; Alkaline CDase 2; haCER2]
uniprot entry name :
ACER2_HUMAN
specificity :
No significant cross-reactivity or interference between this analyte and analogues is observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Undiluted original Human body fluids, tissue homogenates, secretions or feces samples. Assay Type: Sandwich (Quantitative). Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of ACER2 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
ncbi acc num :
NP_001010887.2
ncbi gb acc num :
NM_001010887.2
ncbi mol weight :
31,309 Da
ncbi pathways :
Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Sphingolipid De Novo Biosynthesis Pathway (530750); Sphingolipid Metabolism Pathway (82994); Sphingolipid Metabolism Pathway (119543); Sphingolipid Metabolism Pathway (369); Sphingosine Biosynthesis Pathway (524497); Sphingosine Biosynthesis Pathway (468292); Sphingosine And Sphingosine-1-phosphate Metabolism Pathway (545351)
ncbi summary :
The sphingolipid metabolite sphingosine-1-phosphate promotes cell proliferation and survival, whereas its precursor, sphingosine, has the opposite effect. The ceramidase ACER2 hydrolyzes very long chain ceramides to generate sphingosine (Xu et al., 2006 [PubMed 16940153]).[supplied by OMIM, Jul 2010]
uniprot summary :
ACER2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Unsaturated long-chain ceramides are the best substrates, saturated long-chain ceramides and unsaturated very long-chain ceramides are good substrates, whereas saturated very long-chain ceramides and short-chain ceramides were poor substrates. The substrate preference is D-erythro-C(18:1)-, C(20:1)-, C(20:4)-ceramide D-erythro-C(16:0)-, C(18:0), C(20:0)- ceramide D-erythro-C(24:1)-ceramide D-erythro-C(12:0)- ceramide, D-erythro-C(14:0)-ceramides D-erythro-C(24:0)-ceramide D-erythro-C(6:0)-ceramide. Inhibits the maturation of protein glycosylation in the Golgi complex, including that of integrin beta-1 (ITGB1) and of LAMP1, by increasing the levels of sphingosine. Inhibits cell adhesion by reducing the level of ITGB1 in the cell surface. May have a role in cell proliferation and apoptosis that seems to depend on the balance between sphingosine and sphingosine-1-phosphate. Belongs to the alkaline ceramidase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Lipid Metabolism - sphingolipid; EC 3.5.1.23; Membrane protein, multi-pass; Hydrolase. Chromosomal Location of Human Ortholog: 9p22.1. Cellular Component: Golgi membrane; Golgi apparatus; integral to Golgi membrane. Molecular Function: ceramidase activity. Biological Process: caspase activation; sphingolipid metabolic process; response to retinoic acid; sphingolipid biosynthetic process; positive regulation of cell proliferation; sphingosine biosynthetic process; ceramide metabolic process; negative regulation of cell-matrix adhesion; negative regulation of cell adhesion mediated by integrin
size4 :
10x96-Strip-Wells