catalog number :
MBS931197
products type :
ELISA Kit
products full name :
Human Ig alpha-1 chain C region, IGHA1 ELISA Kit
products short name :
immunoglobulin heavy constant alpha 1
products name syn :
Human Ig alpha-1 chain C region (IGHA1) ELISA kit; FLJ14473; FLJ35065; FLJ35500; FLJ36402; FLJ39698; FLJ40001; FLJ41548; FLJ41552; FLJ41789; FLJ43248; FLJ43594; FLJ44293; FLJ46028; FLJ46621; FLJ46724; FLJ46811; FLJ46824; FLJ90170; IgA1; MGC102857; ; immunoglobulin heavy constant alpha 1
other names :
Ig alpha-1 chain C region, partial; Ig alpha-1 chain C region; immunoglobulin heavy constant alpha 1
products gene name :
IGHA1
other gene names :
IGHA1; IGHA1; IgA1
uniprot entry name :
IGHA1_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human IGHA1. No significant cross-reactivity or interference between human IGHA1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of human Ig alpha-1 chain C region (IGHA1) concentrations in serum, plasma. Detection Range: 0.49 ng/ml - 2000 ng/ml.
other info2 :
Sensitivity: The minimum detectable dose of human IGHA1 is typically less than 0.49 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGHA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGHA1 present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for IGHA1 is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGHA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
37,655 Da
ncbi pathways :
Binding And Uptake Of Ligands By Scavenger Receptors Pathway 771599!!Inflammatory Response Pathway 198766!!Regulation Of Nuclear SMAD2/3 Signaling Pathway 137963!!Scavenging Of Heme From Plasma Pathway 771600
uniprot summary :
Function: Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.