ELISA/assay

Human Mitogen Activated Protein Kinase 8 (MAPK8) ELISA Kit

MBS9311129

96 Strip Wells

600 USD

ELISA Kit

Human Mitogen Activated Protein Kinase 8 (MAPK8) ELISA Kit

Mitogen Activated Protein Kinase 8 (MAPK8)

mitogen-activated protein kinase 8 isoform 5; Mitogen-activated protein kinase 8; mitogen-activated protein kinase 8; MAP kinase 8; JUN N-terminal kinase; c-Jun N-terminal kinase 1; stress-activated protein kinase 1; stress-activated protein kinase 1c; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1

MAPK8

MAPK8; MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2; JNK1; PRKM8; SAPK1; SAPK1C; MAP kinase 8; MAPK 8; SAPK1c

MK08_HUMAN

Human

No significant cross-reactivity or interference between this analyte and analogues is observed.

Samples: Serum, Plasma and Tissue Homogenate. Detection Range: The detection range of this kit is 3.12ng/ml-100ng/ml.

Sensitivity: The sensitivity of this kit is 1.0ng/ml. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of MAPK8 (hereafter termed this analyte) in undiluted original Human serum, plasma and tissue homogenate samples.

513788281

NP_001265477.1

NM_001278548.1

P45983

48,296 Da

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jun 2013]

Function: Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Ref.11 Ref.14 Ref.15 Ref.16 Ref.21 Ref.22 Ref.25 Ref.27JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. Ref.11 Ref.14 Ref.15 Ref.16 Ref.21 Ref.22 Ref.25 Ref.27. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Ref.7. Enzyme regulation: Activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. MAP2K4 shows a strong preference for Tyr-185 while MAP2K7 phosphorylates Tyr-183 preferentially. Inhibited by dual specificity phosphatases, such as DUSP1. Inhibited by SERPINB3. Ref.19. Subunit structure: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP. Forms a complex with MAPK8IP1 and ARHGEF28 . By similarity. Interacts (phosphorylated form) with NFE2; the interaction phosphorylates NFE2 in undifferentiated cells . By similarity. Interacts with NFATC4. Interacts with MECOM; regulates JNK signaling. Interacts with PIN1; this interaction mediates MAPK8 conformational changes leading to the binding of MAPK8 to its substrates. Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.19 Ref.24. Subcellular location: Cytoplasm. Nucleus Ref.26. Domain: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. Post-translational modification: Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2. Ref.13. Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Contains 1 protein kinase domain.

96 Strip Wells

600 USD