Mouse Transmembrane protease serine 6, TMPRSS6 ELISA Kit | MyBioSource MBS931047 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Transmembrane protease serine 6, TMPRSS6 ELISA Kit

catalog :

MBS931047

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS931047

products type :

ELISA Kit

products full name :

Mouse Transmembrane protease serine 6, TMPRSS6 ELISA Kit

products short name :

transmembrane protease, serine 6

products name syn :

Mouse Transmembrane protease serine 6 (TMPRSS6) ELISA kit; RP5-1170K4.5; IRIDA; membrane-bound mosaic serine proteinase matriptase-2; type II transmembrane serine protease 6; transmembrane protease; serine 6

other names :

transmembrane protease serine 6; Transmembrane protease serine 6; transmembrane protease serine 6; matriptase 2; matriptase-2; transmembrane protease, serine 6; type II transmembrane serine protease 6; transmembrane serine protease 6; Matriptase-2

products gene name :

TMPRSS6

other gene names :

Tmprss6; Tmprss6; 1300008A22Rik

uniprot entry name :

TMPS6_MOUSE

reactivity :

Mouse

sequence length :

811

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse TMPRSS6. No significant cross-reactivity or interference between mouse TMPRSS6 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of mouse TMPRSS6 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TMPRSS6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMPRSS6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMPRSS6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMPRSS6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

125656152

ncbi acc num :

NP_082178.2

ncbi gb acc num :

NM_027902.2

uniprot acc num :

Q9DBI0

ncbi mol weight :

90,978 Da

uniprot summary :

TMPRSS6: Serine protease which hydrolyzes a range of proteins including type I collagen, fibronectin and fibrinogen. Can also activate urokinase-type plasminogen activator with low efficiency. May play a specialized role in matrix remodeling processes in liver. Plays a role in the regulation of iron homeostasis, a process involving HAMP. Required to sense iron deficiency and suppress activation of the HAMP promoter. Defects in TMPRSS6 are the cause of iron-refractory iron deficiency anemia (IRIDA); also known as hypochromic microcytic anemia with defect in iron metabolism or hereditary iron-handling disorder or pseudo-iron-deficiency anemia. Key features include congenital hypochromic microcytic anemia, very low mean corpuscular erythrocyte volume, low transferrin saturation, abnormal iron absorption characterized by no hematologic improvement following treatment with oral iron, and abnormal iron utilization characterized by a sluggish, incomplete response to parenteral iron. Mutations leading to abrogation of TMPRSS6 activity are associated with IRIDA due to elevated levels of hepcidin, a negative regulator of plasma iron pool (PubMed:20232450). Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; EC 3.4.21.-; Protease. Cellular Component: membrane; integral to membrane; plasma membrane. Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity. Biological Process: fibrinolysis; iron ion homeostasis; proteolysis; negative regulation of BMP signaling pathway

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage