Mouse Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit | MyBioSource MBS9309033 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit

catalog :

MBS9309033

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS9309033

products type :

ELISA Kit

products full name :

Mouse Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit

products short name :

[Hypoxia Inducible Factor 1 Alpha (HIF1a)]

other names :

[hypoxia-inducible factor 1-alpha; Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor 1-alpha; HIF1 alpha; HIF1-alpha; HIF-1 alpha; HIF-1-alpha; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)<]

products gene name :

[HIF1a]

products gene name syn :

[ARNT-interacting protein,HIF-1-alpha,HIF1-alpha]

other gene names :

[HIF1A; HIF1A; HIF-1-alpha; HIF1-alpha]

uniprot entry name :

HIF1A_BOVIN

reactivity :

Mouse

specificity :

No significant cross-reactivity or interference between this analyte and analogues is observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Duck Body Fluids And Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 6.25pg/ml-200pg/ml. Sensitivity: 1.0pg/ml.

other info2 :

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

products description :

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determine the level of HIF1A (hereafter termed "analyte") in undiluted original Duck body fluids and tissue homogenates. This kit is NOT suitable for assaying non-biological sources of substances.

ncbi gi num :

117935055

ncbi acc num :

NP_776764.2

ncbi gb acc num :

NM_174339.3

uniprot acc num :

Q9XTA5

ncbi mol weight :

92,128 Da

ncbi pathways :

Adipogenesis Pathway (920913); Cellular Response To Hypoxia Pathway (934602); Cellular Responses To Stress Pathway (934601); Circadian Clock Pathway (935298); Disease Pathway (934178); FBXW7 Mutants And NOTCH1 In Cancer Pathway (934296); HIF-1 Signaling Pathway (695207); NOTCH1 Intracellular Domain Regulates Transcription Pathway (934289); Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway (934604); Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway (934605)

uniprot summary :

Function: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia . By similarity. Subunit structure: Interacts with COPS5 subunit of COP9 signalosome complex, leading to the regulation of its stability. Efficient DNA binding requires heterodimerization of an alpha and a beta/ARNT subunit. Interacts with NCOA1, NCOA2, APEX, HSP90 and TSGA10. Interacts with VHL which docks HFA1 to the E3 ubiquitin ligase complex for subsequent destruction. Interaction, via the ODD domain, with the beta domain of VHLL, protects HIF1A from destruction by competing against the destructive targeting initiated by VHL. Interacts with RORA (via the DNA binding domain); the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. Interaction with PSMA7 inhibits the transactivation activity of HIF1A under both normoxic and hypoxia-mimicking conditions . By similarity. Interacts with USP20. Interacts with GNB2L1/RACK1; promotes HIF1A ubiquitination and proteasome-mediated degradation. Interacts with EP300 (via TAZ-type 1 domain); the interaction is stimulated in response to hypoxia and inhibited by CITED2. Interacts with CREBBP (via TAZ-type 1 domain) . By similarity. Interacts (via N-terminus) with USP19 . By similarity. Subcellular location: Cytoplasm . By similarity. Nucleus . By similarity. Note: Cytoplasmic in normoxia, nuclear translocation in response to hypoxia . By similarity. Domain: Contains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID) . By similarity. Post-translational modification: In normoxia, is hydroxylated on Pro-402 and Pro-564 in the oxygen-dependent degradation domain (ODD) by EGLN1/PHD1 and EGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Deubiquitinated by USP20. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization . By similarity.In normoxia, is hydroxylated on Asn-800 by HIF1AN, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation . By similarity.S-nitrosylation of Cys-797 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex . By similarity.Acetylation of Lys-532 by ARD1 increases interaction with VHL and stimulates subsequent proteasomal degradation . By similarity.Requires phosphorylation for DNA-binding. Phosphorylation at Ser-247 by CSNK1D/CK1 represses kinase activity and impairs ARNT binding. Phosphorylation by GSK3-beta and PLK3 promote degradation by the proteasome . By similarity.The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains . By similarity.Sumoylated; with SUMO1 under hypoxia. Sumoylation is enhanced through interaction with RWDD3. Desumoylation by SENP1 leads to increased HIF1A stability and transriptional activity . By similarity. Sequence similarities: Contains 1 bHLH (basic helix-loop-helix) domain.Contains 1 PAC (PAS-associated C-terminal) domain.Contains 2 PAS (PER-ARNT-SIM) domains.

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

680

size3 :

5x96-Strip-Wells

price3 :

3100

size4 :

10x96-Strip-Wells

price4 :

6095

more info or order :

MyBioSource product webpage