Mouse Ectodysplasin-A, EDA ELISA Kit | MyBioSource MBS930835 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Ectodysplasin-A, EDA ELISA Kit

catalog :

MBS930835

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS930835

products type :

ELISA Kit

products full name :

Mouse Ectodysplasin-A, EDA ELISA Kit

products short name :

ectodysplasin A

products name syn :

Mouse Ectodysplasin-A (EDA) ELISA kit; RP11-351K23.1; ED1; ED1-A1; ED1-A2; EDA1; EDA2; HED; XHED; XLHED; X-linked anhidroitic ectodermal dysplasia protein; ectodysplasin A

other names :

ectodysplasin-A isoform 2; Ectodysplasin-A; ectodysplasin-A; EDA protein homolog; ectodysplasin-A; EDA protein homolog; Tabby proteinCleaved into the following 2 chains:Ectodysplasin-A, membrane form; Ectodysplasin-A, secreted form

products gene name :

EDA

other gene names :

Eda; Eda; Ta; Ed1; HED; EDA1; XLHED; tabby; Eda-A1; Eda-A2; Ed1; Ta

uniprot entry name :

EDA_MOUSE

reactivity :

Mouse

sequence length :

389

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse EDA. No significant cross-reactivity or interference between mouse EDA and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: 3.9 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EDA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EDA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EDA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EDA bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

295789018

ncbi acc num :

NP_001171408.1

ncbi gb acc num :

NM_001177937.1

uniprot acc num :

O54693

ncbi mol weight :

41,603 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460)

uniprot summary :

EDA: Seems to be involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Isoform 1 binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor XEDAR. Defects in EDA are the cause of ectodermal dysplasia type 1 (ED1); also known as Christ-Siemens-Touraine syndrome or X-linked hypohidrotic ectodermal dysplasia (XLHED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ED1 is a disease characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. ED1 is the most common form of over 150 clinically distinct ectodermal dysplasias. Defects in EDA are the cause of tooth agenesis selective X-linked type 1 (STHAGX1). A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth). Belongs to the tumor necrosis factor family. 8 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc. Cellular Component: endoplasmic reticulum membrane; collagen; membrane; integral to plasma membrane; apical part of cell; integral to membrane; plasma membrane; extracellular region. Molecular Function: tumor necrosis factor receptor binding. Biological Process: skin development; positive regulation of I-kappaB kinase/NF-kappaB cascade; pigmentation; hair follicle development; cell-matrix adhesion; multicellular organismal development; immune response; positive regulation of NF-kappaB import into nucleus; gene expression; cell differentiation; odontogenesis of dentine-containing teeth

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage