ELISA/assay

Rat Platelet Derived Growth Factor Subunit A (PDGFA) ELISA Kit

MBS9307869

48-Strip-Wells

470 USD

ELISA Kit

Rat Platelet Derived Growth Factor Subunit A (PDGFA) ELISA Kit

[Platelet Derived Growth Factor Subunit A (PDGFA)]

[platelet-derived growth factor subunit A isoform 1 preproprotein; Platelet-derived growth factor subunit A; platelet-derived growth factor subunit A; PDGF-1; PDGF A-chain; PDGF subunit A; platelet-derived growth factor A chain; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha isoform 2 preproprotein; platelet-derived growth factor alpha polypeptide; PDGF-1; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide]

[PDGFA]

[PDGF subunit A,PDGF-1,Pdgfa,Platelet-derived growth factor A chain,Platelet-derived growth factor alpha polypeptide,Platelet-derived growth factor subunit A,Rpa1]

[PDGFA; PDGFA; PDGF1; PDGF-A; PDGF1; PDGF subunit A]

PDGFA_HUMAN

Rat

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Body fluids, tissue homogenates, secretions or feces samples. Assay Type: Quantitative Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of PDGFA (hereafter termed "analyte") in undiluted original Rat body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

77695917

NP_002598.4

NM_002607.5

P04085

24,043 Da

Adaptive Immune System Pathway (366160); Ceramide Signaling Pathway (138023); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250)

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]

PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Cell cycle regulation; Cytokine. Chromosomal Location of Human Ortholog: 7p22. Cellular Component: Golgi membrane; extracellular space; cell surface; microvillus; endoplasmic reticulum lumen; extracellular region. Molecular Function: collagen binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding. Biological Process: skin development; extracellular matrix organization and biogenesis; wound healing; nerve growth factor receptor signaling pathway; negative chemotaxis; platelet-derived growth factor receptor signaling pathway; cell projection biogenesis; response to estradiol stimulus; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; platelet degranulation; regulation of smooth muscle cell migration; hair follicle development; cell-cell signaling; positive regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to wounding; angiogenesis; regulation of peptidyl-tyrosine phosphorylation; inner ear development; epidermal growth factor receptor signaling pathway; response to drug; platelet activation; response to inorganic substance; phosphoinositide-mediated signaling; response to retinoic acid; fibroblast growth factor receptor signaling pathway; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; cell activation; organ morphogenesis; positive regulation of cell division; regulation of actin cytoskeleton organization and biogenesis; response to hypoxia; innate immune response; actin cytoskeleton organization and biogenesis; blood coagulation; alveolus development; positive regulation of DNA replication; positive regulation of cell migration

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095