ELISA/assay

Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, ENPP2 ELISA Kit

MBS930753

48-Strip-Wells

510 USD

ELISA Kit

Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, ENPP2 ELISA Kit

ectonucleotide pyrophosphatase/phosphodiesterase 2

Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (ENPP2) ELISA kit; ATX; ATX-X; AUTOTAXIN; FLJ26803; LysoPLD; NPP2; PD-IALPHA; PDNP2; autotaxin; autotaxin-t; phosphodiesterase I/nucleotide pyrophosphatase 2; plasma lysophospholipase D; ectonucleotide pyrophosphatase/phosphodiesterase 2

ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform 2 preproprotein; Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; autotaxin-t; plasma lysophospholipase D; extracellular lysophospholipase D; phosphodiesterase I/nucleotide pyrophosphatase 2; ectonucleotide pyrophosphatase/phosphodiesterase 2; Autotaxin; Extracellular lysophospholipase D; LysoPLD

ENPP2

ENPP2; ENPP2; ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA; ATX; PDNP2; E-NPP 2; LysoPLD

ENPP2_HUMAN

Human

863

This assay has high sensitivity and excellent specificity for detection of Human ENPP2. No significant cross-reactivity or interference between Human ENPP2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and tissue homogenates. Assay Type: Competitive. Detection Range: 50ng/ml-2000 ng/ml. Sensitivity: 50ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 2: chemokine. Protein Biological Process 3: Chemotaxis

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ENPP2. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for ENPP2. The competitive inhibition reaction is launched between with pre-coated ENPP2 and ENPP2 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of ENPP2 in the samples. The color development is stopped and the intensity of the color is measured.

91823602

NP_001035181.1

NM_001040092.2

Q13822

98,994 Da

Ether Lipid Metabolism Pathway (82990); Ether Lipid Metabolism Pathway (365)

The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2008]

ENPP2: Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.4.39; Motility/polarity/chemotaxis; Secreted, signal peptide; Lipid Metabolism - ether lipid; Secreted; Phosphodiesterase. Chromosomal Location of Human Ortholog: 8q24.1. Cellular Component: extracellular space; integral to plasma membrane; plasma membrane. Molecular Function: nucleotide diphosphatase activity; phosphodiesterase I activity; nucleic acid binding; hydrolase activity; zinc ion binding; calcium ion binding; transcription factor binding; alkylglycerophosphoethanolamine phosphodiesterase activity; scavenger receptor activity; polysaccharide binding; lysophospholipase activity. Biological Process: G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; phospholipid catabolic process; immune response; cell motility; chemotaxis; regulation of angiogenesis; phosphate metabolic process; regulation of cell migration

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800