Rat Angiopoietin-related protein 4, ANGPTL4 ELISA Kit | MyBioSource MBS930738 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Angiopoietin-related protein 4, ANGPTL4 ELISA Kit

catalog :

MBS930738

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS930738

products type :

ELISA Kit

products full name :

Rat Angiopoietin-related protein 4, ANGPTL4 ELISA Kit

products short name :

angiopoietin-like 4

products name syn :

Rat Angiopoietin-related protein 4 (ANGPTL4) ELISA kit; ANGPTL2; ARP4; FIAF; HFARP; NL2; PGAR; pp1158; PPARG angiopoietin related protein; angiopoietin-like 4 protein; fasting-induced adipose factor; hepatic angiopoietin-related protein; hepatic fibrinogen/a; angiopoietin-like 4

other names :

angiopoietin-related protein 4; Angiopoietin-related protein 4; angiopoietin-related protein 4; HFARP; angiopoietin-like protein 4; hepatic fibrinogen/angiopoietin-related protein; angiopoietin-like 4; Angiopoietin-like protein 4; Hepatic fibrinogen/angiopoietin-related protein; HFARP

products gene name :

ANGPTL4

other gene names :

Angptl4; Angptl4; HFARP

uniprot entry name :

ANGL4_RAT

reactivity :

Rat

sequence length :

405

specificity :

This assay has high sensitivity and excellent specificity for detection of rat ANGPTL4. No significant cross-reactivity or interference between rat ANGPTL4 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, bronchoalveolar lavage fluid, urine, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: The minimum detectable dose of rat ANGPTL4 is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANGPTL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANGPTL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANGPTL4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANGPTL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

40018598

ncbi acc num :

NP_954546.1

ncbi gb acc num :

NM_199115.2

uniprot acc num :

Q6TMA8

ncbi mol weight :

44,951 Da

ncbi pathways :

Developmental Biology Pathway (1009870); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (1010691); Metabolism Pathway (1010675); Metabolism Of Lipids And Lipoproteins Pathway (1010682); PPAR Signaling Pathway (83434); PPAR Signaling Pathway (450); PPARA Activates Gene Expression Pathway (1010711); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (1010710); Transcriptional Regulation Of White Adipocyte Differentiation Pathway (1009920)

ncbi summary :

a circulating protein which causes an increase in plasma very low density lipoprotein by inhibition of lipoprotein lipase activity [RGD, Feb 2006]

uniprot summary :

Function: Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage