ELISA/assay

Human Prominin-1, PROM1 ELISA Kit

MBS930646

48-Strip-Wells

510 USD

ELISA Kit

Human Prominin-1, PROM1 ELISA Kit

prominin 1

Human Prominin-1 (PROM1) ELISA kit; AC133; CD133; CORD12; MCDR2; MSTP061; PROML1; RP41; STGD4; hProminin; hematopoietic stem cell antigen; prominin-like 1; prominin 1

prominin-1 isoform 2; Prominin-1; prominin-1; hProminin; antigen AC133; prominin-like protein 1; hematopoietic stem cell antigen; prominin 1; Antigen AC133; Prominin-like protein 1; CD_antigen: CD133

PROM1

PROM1; PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061; PROML1; MSTP061

PROM1_HUMAN

Human

856

This assay has high sensitivity and excellent specificity for detection of human PROM1. No significant cross-reactivity or interference between human PROM1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0. 312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of human PROM1 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PROM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PROM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROM1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

224994189

NP_001139319.1

NM_001145847.1

O43490

97,202 Da

Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

CD133: Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. Interacts with CDHR1 and with actin filaments. Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells. Expressed in adult retina by rod and cone photoreceptor cells. Belongs to the prominin family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cell surface; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 4p15.32. Cellular Component: extracellular space; stereocilium; photoreceptor outer segment; cell surface; endoplasmic reticulum; microvillus membrane; integral to plasma membrane; apical plasma membrane; ER-Golgi intermediate compartment; plasma membrane; vesicle; brush border. Molecular Function: actinin binding; protein binding; cadherin binding. Biological Process: photoreceptor cell maintenance; retina morphogenesis in camera-type eye. Disease: Macular Dystrophy, Retinal, 2; Stargardt Disease 4; Retinitis Pigmentosa 41; Cone-rod Dystrophy 12

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800