ELISA/assay

Human Carbonic anhydrase 7, CA7 ELISA Kit

MBS930609

48-Strip-Wells

510 USD

ELISA Kit

Human Carbonic anhydrase 7, CA7 ELISA Kit

carbonic anhydrase VII

Human Carbonic anhydrase 7 (CA7) ELISA kit; CAVII; carbonic anhydrase 7; carbonic dehydratase VII; carbonic anhydrase VII

carbonic anhydrase 7 isoform 2; Carbonic anhydrase 7; carbonic anhydrase 7; CA-VII; carbonic dehydratase VII; carbonate dehydratase VII; carbonic anhydrase VII; Carbonate dehydratase VII; Carbonic anhydrase VII; CA-VII

CA7

CA7; CA7; CAVII; CA-VII

CAH7_HUMAN

Human

208

This assay has high sensitivity and excellent specificity for detection of human CA7. No significant cross-reactivity or interference between human CA7 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 125 pg/ml -8000 pg/ml. Sensitivity: The minimum detectable dose of human CA7 is typically less than 31.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CA7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CA7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CA7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CA7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

62240989

NP_001014435.1

NM_001014435.1

P43166

29,658 Da

Metabolism Pathway (477135); Nitrogen Metabolism Pathway (83025); Nitrogen Metabolism Pathway (416); Reversible Hydration Of Carbon Dioxide Pathway (645352)

Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. The cytosolic protein encoded by this gene is predominantly expressed in the salivary glands. Alternative splicing in the coding region results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

CA7: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase. Chromosomal Location of Human Ortholog: 16q22.1. Cellular Component: cytosol. Molecular Function: carbonate dehydratase activity; zinc ion binding. Biological Process: bicarbonate transport; positive regulation of cellular pH reduction; positive regulation of synaptic transmission, GABAergic; one-carbon compound metabolic process

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800