ELISA/assay

Porcine secreted protein acidic and rich in cysteine, SPARC ELISA Kit

MBS9304797

48-Strip-Wells

435 USD

ELISA Kit

Porcine secreted protein acidic and rich in cysteine, SPARC ELISA Kit

secreted protein acidic and rich in cysteine, SPARC

SPARC; SPARC; SPARC; BM-40; osteonectin; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin); Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine

SPARC

SPARC; SPARC; ON; ON; BM-40; ON

SPRC_HUMAN

Porcine

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma and Tissue Homogenate. Assay Type: Sandwich. Detection Range: 25 ng/ml - 800 ng/ml. Sensitivity: 2.0 ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of SPARC (hereafter termed this analyte) in undiluted original Porcine serum, plasma and tissue homogenate samples.

48145715

CAG33080.1

P09486

34,632 Da

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); ECM Proteoglycans Pathway (833812); Extracellular Matrix Organization Pathway (576262); Hemostasis Pathway (106028); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048); Scavenging By Class H Receptors Pathway (833817); Senescence And Autophagy Pathway (198780); TGF-beta Receptor Signaling Pathway (198774)

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. [provided by RefSeq, Dec 2011]

SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 5q31.3-q32. Cellular Component: extracellular space; platelet alpha granule membrane; nuclear matrix; cell surface; mitochondrion; cytoplasm; extracellular region; plasma membrane; basement membrane. Molecular Function: collagen binding; protein binding; extracellular matrix binding; calcium ion binding. Biological Process: receptor-mediated endocytosis; response to gravity; platelet activation; response to peptide hormone stimulus; ossification; extracellular matrix organization and biogenesis; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; platelet degranulation; response to ethanol; response to cadmium ion; response to cytokine stimulus; negative regulation of endothelial cell proliferation; response to lead ion; regulation of cell morphogenesis; response to calcium ion; blood coagulation; inner ear development; lung development. Disease: Osteogenesis Imperfecta, Type Xvii

48-Strip-Wells

435 USD

96-Strip-Wells

600