ELISA/assay

Canine Proinsulin, PI ELISA Kit

MBS9304712

48-Strip-Wells

470 USD

ELISA Kit

Canine Proinsulin, PI ELISA Kit

[Proinsulin, PI]

[proinsulin; Insulin; insulin; proinsulin; preproinsulin; insulin]

[PI]

[INS; INS; ILPR; IRDN; IDDM2; MODY10]

INS_HUMAN

Canine

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Undiluted original Canine body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances. Assay Type: Sandwich. Detection Range: 1.25 pmol/L - 40 pmol/L. Sensitivity: 0.1 pmol/L.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, NOT for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of PINS (hereafter termed "analyte") in undiluted original Canine body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

59036749

AAW83741.1

P01308

11,981 Da

AGE/RAGE Pathway (698754); ATF-2 Transcription Factor Network Pathway (138006); Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Amyloids Pathway (366238); Arf6 Trafficking Events Pathway (137954); Cardiac Progenitor Differentiation Pathway (712094); Developmental Biology Pathway (477129); Disease Pathway (530764)

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family. Protein type: Secreted; Hormone; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: extracellular space; endoplasmic reticulum lumen; Golgi lumen; extracellular region. Molecular Function: identical protein binding; insulin-like growth factor receptor binding; protein binding; protease binding; hormone activity; insulin receptor binding. Biological Process: positive regulation of nitric oxide biosynthetic process; glucose homeostasis; positive regulation of vasodilation; activation of NF-kappaB transcription factor; positive regulation of glucose import; negative regulation of protein oligomerization; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; positive regulation of nitric-oxide synthase activity; activation of protein kinase B; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; regulation of amino acid metabolic process; negative regulation of protein catabolic process; positive regulation of cell differentiation; negative regulation of glycogen catabolic process; wound healing; positive regulation of glycogen biosynthetic process; positive regulation of cellular protein metabolic process; glucose transport; regulation of protein secretion; regulation of transcription, DNA-dependent; positive regulation of MAPKKK cascade; negative regulation of lipid catabolic process; negative regulation of vasodilation; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; negative regulation of fatty acid metabolic process; alpha-beta T cell activation; endocrine pancreas development; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; energy reserve metabolic process; insulin receptor signaling pathway; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cytokine secretion; positive regulation of cell migration. Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095